dc.contributor.author | Coutts, Robert H.A. | |
dc.contributor.author | Wood, K.R. | |
dc.date.accessioned | 2014-02-19T15:58:59Z | |
dc.date.available | 2014-02-19T15:58:59Z | |
dc.date.issued | 1977-05-01 | |
dc.identifier.citation | Coutts , R H A & Wood , K R 1977 , ' Improved isolation and culture methods for cucumber mesophyll protoplasts ' , Plant Science Letters , vol. 9 , no. 1 , pp. 45-51 . https://doi.org/10.1016/0304-4211(77)90009-8 | |
dc.identifier.issn | 0304-4211 | |
dc.identifier.other | PURE: 1957231 | |
dc.identifier.other | PURE UUID: d68c3ca4-c146-448d-a115-a51b0dca17a0 | |
dc.identifier.other | Scopus: 0006461517 | |
dc.identifier.uri | http://hdl.handle.net/2299/12863 | |
dc.description.abstract | Improved methods for the isolation of large numbers of viable protoplasts from cotyledons and first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China are described. Short-term isolation methods (STM), and long-term isolation methods (LTM), using a variety of commercial enzymes are outlined. Liquid culture and subsequent solid culture of isolated first leaf protoplasts resulted in sustained cell division and eventual compact callus formation, which has been induced to form roots. No shoot or whole plant regeneration has, as yet, been demonstrated | en |
dc.format.extent | 7 | |
dc.language.iso | eng | |
dc.relation.ispartof | Plant Science Letters | |
dc.title | Improved isolation and culture methods for cucumber mesophyll protoplasts | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=0006461517&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | https://doi.org/10.1016/0304-4211(77)90009-8 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |