Show simple item record

dc.contributor.authorSewell, Thomas
dc.contributor.authorJ Hawkins, Nichola
dc.contributor.authorStotz, Henrik
dc.contributor.authorHuang, Yongju
dc.contributor.authorL Kelly, Steven
dc.contributor.authorE Kelly, Diane
dc.contributor.authorFraaije, Bart
dc.contributor.authorFitt, Bruce
dc.date.accessioned2017-11-28T18:03:56Z
dc.date.available2017-11-28T18:03:56Z
dc.date.issued2017-11-20
dc.identifier.citationSewell , T , J Hawkins , N , Stotz , H , Huang , Y , L Kelly , S , E Kelly , D , Fraaije , B & Fitt , B 2017 , ' Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase ' , Scientific Reports , vol. 7 , 15849 . https://doi.org/10.1038/s41598-017-15545-9
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/2299/19563
dc.descriptionOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
dc.description.abstractLanosterol 14-α demethy lase is a key enzyme intermediating the biosynthesis of ergosterol in fungi, and the target of azole fungicides. Studies have suggested that Leptosphaeria maculans and L. biglobosa, the causal agents of phoma stem canker on oilseed rape, differ in their sensitivity to some azoles, which could be driving pathogen frequency change in crops. Here we used CYP51 protein modelling and heterologous expression to determine whether there are interspecific differences at the target-site level. Moreover, we provide an example of intrinsic sensitivity differences exhibited by both Leptosphaeria spp. in vitro and in planta. Comparison of homologous protein models identified highly conserved residues, particularly at the azole binding site, and heterologous expression of LmCYP51B and LbCYP51B, with fungicide sensitivity testing of the transformants, suggests that both proteins are similarly sensitive to azole fungicides flusilazole, prothioconazole-desthio and tebuconazole. Fungicide sensitivity testing on isolates shows that they sometimes have a minor difference in sensitivity in vitro and in planta. These results suggest that azole fungicides remain a useful component of integrated phoma stem canker control in the UK due to their effectiveness on both Leptosphaeria spp. Other factors, such as varietal resistance or climate, may be driving observed frequency changes between species.en
dc.format.extent2308725
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.titleAzole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylaseen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionWeight and Obesity Research Group
dc.description.statusPeer reviewed
dc.identifier.urlhttp://rdcu.be/yUEw
rioxxterms.versionofrecord10.1038/s41598-017-15545-9
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record