Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
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Author
Uguru, Gabriel C
Mondhe, Madhav
Goh, Shan
Hesketh, Andrew
Bibb, Mervyn J
Good, Liam
Stach, James E M
Attention
2299/20576
Abstract
We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.