Show simple item record

dc.contributor.authorSchmidt, E. K.
dc.contributor.authorLiebermann, T.
dc.contributor.authorKreiter, M.
dc.contributor.authorJonczyk, A.
dc.contributor.authorNaumann, R.
dc.contributor.authorOffenhausser, A.
dc.contributor.authorNeumann, E.
dc.contributor.authorKukol, A.
dc.contributor.authorMaelicke, A.
dc.contributor.authorKnoll, W.
dc.date.accessioned2011-10-18T12:01:22Z
dc.date.available2011-10-18T12:01:22Z
dc.date.issued1998-09-15
dc.identifier.citationSchmidt , E K , Liebermann , T , Kreiter , M , Jonczyk , A , Naumann , R , Offenhausser , A , Neumann , E , Kukol , A , Maelicke , A & Knoll , W 1998 , ' Incorporation of the acetylcholine receptor dimer from Torpedo californica in a peptide supported lipid membrane investigated by surface plasmon and fluorescence spectroscopy ' , Biosensors & Bioelectronics , vol. 13 , no. 6 , pp. 585-591 . https://doi.org/10.1016/S0956-5663(98)00013-X
dc.identifier.issn0956-5663
dc.identifier.urihttp://hdl.handle.net/2299/6704
dc.descriptionFull text of this article is not available in the UHRA
dc.description.abstractThe dimer species (M-r 580 000) of the nicotinic acetylcholine receptor, isolated from the electric organ of Torpedo californica, was incorporated into a thiopeptide supported lipid bilayer. The incorporation was achieved by fusion of liposomes with reconstituted receptor onto a gold-supported thiopeptide lipid monolayer. Surface plasmon resonance spectroscopy (SPS) was used to monitor in real time the fusion process as well as the specific binding of the antagonist cu-bungarotoxin. A recently developed extension of SPS offering enhanced sensitivity and specificity, surface plasmon fluorescence spectroscopy (SPFS), was then used to monitor subsequent binding of the monoclonal WF6 and polyclonal antibody, respectively. The latter was fluorescence labeled with Cy5. The different binding assays indicate the successful incorporation of the receptor in the lipid bilayer. (C) 1998 Elsevier Science S.A. All rights reserved.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofBiosensors & Bioelectronics
dc.subjectacetylcholine receptor from Torpedo californica
dc.subjectincorporation
dc.subjectpeptide supported lipid bilayer
dc.subjectsurface plasmon resonance spectroscopy
dc.subjectsurface plasmon fluorescence spectroscopy
dc.subjectbinding assays
dc.subjectalpha-bungarotoxin
dc.subjectmonoclonal antibody WF6
dc.subjectsecondary polyclonal antibody
dc.subjectCYTOCHROME-C-OXIDASE
dc.subjectION-CHANNEL
dc.subjectBILAYERS
dc.subjectBINDING
dc.subjectRESONANCE
dc.subjectBIOSENSOR
dc.subjectRESPONSES
dc.subjectPROTEINS
dc.subjectLAYERS
dc.titleIncorporation of the acetylcholine receptor dimer from Torpedo californica in a peptide supported lipid membrane investigated by surface plasmon and fluorescence spectroscopyen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionCentre for Research in Mechanisms of Disease and Drug Discovery
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Future Societies Research
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1016/S0956-5663(98)00013-X
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record