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Browsing by Author "Freeman, J."
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The changing epidemiology of Clostridium difficile infections
Freeman, J.; Bauer, M. P.; Baines, Simon D.; Corver, J.; Fawley, W. N.; Goorhuis, B.; Kuijper, E. J.; Wilcox, M. H. (2010)The epidemiology of Clostridium difficile infection (CDI) has changed dramatically during this millennium. Infection rates have increased markedly in most countries with detailed surveillance data. There have been clear ... -
Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays
Calderon, C.; Ward, Elaine; Freeman, J.; McCartney, A. (2002-02)Conventional methods for detecting airborne fungal spores rely on either optical identification or culturing and can be time consuming or unreliable. A method for purifying DNA from conventional spore samplers and detecting ... -
Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays
Calderon, C.; Ward, Elaine; Freeman, J.; Foster, S. J.; McCartney, H. A. (2002-06)The potential use of DNA-based methods for detecting airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae, both damaging pathogens of oilseed rape, was investigated. A method for purifying DNA from spores ... -
Efficacy of novel antimicrobial agent SMT-19969 (SMT) against simulated Clostridium difficile infection in an in vitro human gut model
Baines, Simon D.; Freeman, J.; Huscroft, Grace S.; Todhunter, Sharie L.; Wilcox, M. H.; Vickers, R. (2011-09)Background: C. difficile infection (CDI) is the leading cause of nosocomial diarrhoea. Current CDI therapy is limited to metronidazole (M) or vancomycin. Recent evidence suggests reduced M efficacy and emergence of reduced ... -
Evaluation of the effect of oritavancin on Clostridium difficile spore germination, outgrowth and recovery
Chilton, C. H.; Freeman, J.; Baines, Simon D.; Crowther, G. S.; Nicholson, S.; Wilcox, M. H. (2013)OBJECTIVES: Previous work suggests oritavancin may be inhibitory to Clostridium difficile spores. We have evaluated the effects of oritavancin exposure on C. difficile spore germination, outgrowth and recovery. METHODS: ... -
Gaeumannomyces graminis, the take-all fungus and its relatives
Freeman, J.; Ward, Elaine (2004-07)Take-all, caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat worldwide. Many years of intensive research, reflected by the large volume of literature on take-all, has led ... -
In vitro activity of cadazolid against clinically relevant Clostridium difficile isolates and in an in vitro gut model of C. difficile infection
Chilton, C. H.; Crowther, G. S.; Baines, Simon D.; Todhunter, S. L.; Freeman, J.; Locher, H. H.; Athanasiou, A.; Wilcox, M. H. (2013-10-14)Abstract Objectives We investigated the in vitro activity of cadazolid against 100 Clostridium difficile isolates and its efficacy in a simulated human gut model of C. difficile infection (CDI). Methods MICs of cadazolid, ... -
In vitro susceptibility of Clostridium difficile to SMT19969 and comparators, as well as the killing kinetics and post-antibiotic effects of SMT19969 and comparators against C. difficile
Corbett, D.; Wise, A.; Birchall, S.; Warn, P.; Baines, Simon D.; Crowther, G.; Freeman, J.; Chilton, C. H.; Vernon, J.; Wilcox, M. H.; Vickers, R. J. (2015-06-01)OBJECTIVES: SMT19969 is a novel antimicrobial under clinical development for the treatment of Clostridium difficile infection (CDI). The objective was to determine the comparative susceptibility of 82 C. difficile clinical ... -
Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici
Freeman, J.; Ward, Elaine; Gutteridge, R.J.; Bateman, Geoffrey L. (2005-10)Field isolates (n = 144) of the wheat take-all fungus Gaeumannomyces graminis var. tritici (Ggt) were tested for sensitivity to silthiofam, a take-all-specific fungicide used as a seed treatment, and identified as A- or ... -
A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum
Freeman, J.; Ward, Elaine; Calderon, C.; McCartney, A. (2002-11)The development of a polymerase chain reaction (PCR) assay for the detection of inoculum of the plant pathogenic fungus Sclerotinia sclerotiorum is described. The PCR primers were designed using nuclear ribosomal DNA ... -
Short-term genomic stability of Clostridium difficile within patients and a gut model
Eyre, D. W.; Ip, C.; Harding, R. M.; Freeman, J.; Baines, Simon D.; Fawley, W. N.; Walker, S.D.; Crook, D. W.; Peto, T. E. A.; Wilcox, M. H. (2011-09)Background: Clostridium difficile (CD) whole genome sequencing (WGS) has potential to identify related isolates for transmission studies, even among otherwise indistinguishable strains, but depends on understanding the ... -
SMT19969 as a treatment for Clostridium difficile infection : an assessment of antimicrobial activity using conventional susceptibility testing and an in vitro gut model
Baines, Simon D.; Crowther, G. S.; Freeman, J.; Todhunter, S.; Vickers, R.; Wilcox, M. H. (2015-01-01)OBJECTIVES: We investigated the efficacy of the novel antimicrobial agent SMT19969 in treating simulated Clostridium difficile infection using an in vitro human gut model. METHODS: Concentrations of the predominant cultivable ... -
SMT19969 – a novel antibiotic for C. difficile : Clostridium difficile growth inhibition, spectrum of activity and resistance development
Vickers, R.; Tinsley, J.; Storer, R.; Wilson, F.; Dorgan, C.; Wren, S.; Wilcox, M. H.; Baines, Simon D.; Freeman, J. (2011-09)Background: C.difficile infection (CDI) is now established as a major healthcare issue. However, therapy options are limited and recurrent disease remains a significant problem. SMT19969 is the lead compound from a novel ...