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Browsing by Author "Opperman, Charles H."
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Interactions between bacteria and plant-parasitic nematodes : now and then
Bird, D. M.; Opperman, Charles H.; Davies, Keith (2003-09-30)Based on genome-to-genome analyses of gene sequences obtained from plant-parasitic, root-knot nematodes (Meloidogyne spp.), it seems likely that certain genes have been derived from bacteria by horizontal gene transfer. ... -
A method for release and multiple strand amplification of small quantities of DNA from endospores of the fastidious bacterium Pasteuria penetrans
Mauchline, Tim H.; Mohan, Sharad; Schaff, J. E.; Opperman, Charles H.; Kerry, Brian R.; Davies, Keith; Hirsch, Penny R. (2010-05)Aims: To establish a reliable protocol to extract DNA from Pasteuria penetrans endospores for use as template in multiple strand amplification, thus providing sufficient material for genetic analyses. To develop a highly ... -
Phylogenetic analysis of Pasteuria penetrans by use of multiple genetic loci
Charles, L.; Carbone, I.; Davies, Keith; Bird, D.; Burke, M.; Kerry, Brian R.; Opperman, Charles H. (2005-08)Pasteuria penetrans is a gram-positive, endospore-forming eubacterium that apparently is a member of the Bacillus-Clostridium clade. It is an obligate parasite of root knot nematodes (Meloidogyne spp.) and preferentially ... -
Re-evaluation of the life-cycle of the nematode-parasitic bacterium Pasteuria penetrans in root-knot nematodes, Meloidogyne spp.
Davies, Keith G.; Rowe, Janet; Manzanilla-Lopez, Rosa; Opperman, Charles H. (2011)Comparisons of the growth of Pasteuria penetrans in adult root-knot nematode females infected with P. penetrans dissected from the roots of tomato plants were undertaken using bright-field and scanning electron microscopy. ... -
Structural analysis of divalent metals binding to the Bacillus subtilis response regulator Spo0F : the possibility for In vitro metalloregulation in the initiation of sporulation
Kojetin, D. J.; Thompson, R. J.; Benson, L. M.; Naylor, S.; Waterman, J.; Davies, Keith; Opperman, Charles H.; Stephenson, Keith; Hoch, J. A.; Cavanagh, J. (2005-10)The presence of a divalent metal ion in a negatively charged aspartic acid pocket is essential for phosphorylation of response regulator proteins. Here, we present metal binding studies of the Bacillus subtilis response ...