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dc.contributor.authorSmith, Madeleine J.
dc.contributor.authorAdams, Michael J.
dc.contributor.authorWard, Elaine
dc.date.accessioned2013-06-17T11:00:44Z
dc.date.available2013-06-17T11:00:44Z
dc.date.issued2013-02
dc.identifier.citationSmith , M J , Adams , M J & Ward , E 2013 , ' Ribosomal DNA analyses reveal greater sequence variation in Polymyxa species than previously thought and indicate the possibility of new ribotype-host-virus associations. ' Environmental Microbiology Reports , vol 5 , no. 1 , pp. 143-150 . DOI: 10.1111/1758-2229.12026en
dc.identifier.issn1758-2229
dc.identifier.otherPURE: 1300425
dc.identifier.otherPURE UUID: f9de28f1-074c-4eb8-ad7b-ddc821ef2274
dc.identifier.otherScopus: 84873302804
dc.identifier.urihttp://hdl.handle.net/2299/10780
dc.description.abstractPolymyxa species transmit viruses to many important crops. They are poorly-understood obligate 29 parasites occupying a distinct position in the Tree of Life. To better understand the potential for 30 spread of Polymyxa-vectored diseases, ribosomal DNA was analysed from isolates covering a wide 31 range of geographic locations, virus associations and hosts. ITS2 structure analysis indicated that P. 32 graminis isolates could represent many species and there was more sequence variation within the 33 known subgroups (ribotypes) than previously described. In cereal crops and soils from temperate 34 climates Polymyxa isolates were usually ribotype I or II, but their host specificities or preferences 35 were unclear. For the first time, there was evidence that ribotype I (in addition to ribotype II) could 36 transmit SBWMV/ SBCMV. Different ribotypes often occurred together in the same soil or plant. 37 New hosts were identified for particular ribotypes, including the first detection of the sugar beet38 infecting P. betae, in wheat. Unexpectedly, ribotype III-like sequences, usually restricted to crops 39 in the tropics, were found in wheat from the USA. P. betae isolates showed limited variation (≤2%) 40 and the recent change in susceptibility of sugar beet varieties to BNYVV in the USA is unlikely to 41 be due to changes in P. betae.en
dc.language.isoeng
dc.relation.ispartofEnvironmental Microbiology Reportsen
dc.rightsen
dc.titleRibosomal DNA analyses reveal greater sequence variation in Polymyxa species than previously thought and indicate the possibility of new ribotype-host-virus associations.en
dc.typeArticleen
dc.contributor.institutionCrop and Environmental Protectionen
dc.contributor.institutionDepartment of Human and Environmental Sciencesen
dc.contributor.institutionHealth & Human Sciences Research Instituteen
dc.contributor.institutionGeography, Environment and Agricultureen
dc.contributor.institutionAgriculture, Veterinary and Food Sciencesen
dc.identifier.doihttp://dx.doi.org/10.1111/1758-2229.12026
dc.description.statusPeer revieweden
herts.preservation.rarelyaccessedtrue


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