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dc.contributor.authorEleftherianos, I.
dc.contributor.authorFoster, S. P.
dc.contributor.authorWilliamson, M. S.
dc.contributor.authorDenholm, Ian
dc.date.accessioned2013-12-16T14:30:13Z
dc.date.available2013-12-16T14:30:13Z
dc.date.issued2008-04
dc.identifier.citationEleftherianos , I , Foster , S P , Williamson , M S & Denholm , I 2008 , ' Characterization of the M918T sodium channel gene mutation associated with strong resistance to pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer) ' , Bulletin of entomological research , vol. 98 , no. 2 , pp. 183-191 . https://doi.org/10.1017/S0007485307005524
dc.identifier.issn0007-4853
dc.identifier.otherPURE: 1936698
dc.identifier.otherPURE UUID: e85104a8-bfbe-4de4-a8f9-73750fdb1eb9
dc.identifier.otherWOS: 000259233200008
dc.identifier.otherScopus: 40449125203
dc.identifier.otherORCID: /0000-0001-9797-874X/work/35873716
dc.identifier.urihttp://hdl.handle.net/2299/12339
dc.description.abstractRecent advances in the characterisation of insect sodium channel gene sequences have identified a small number of point mutations within the channel protein that are implicated in conferring target-site resistance to pyrethroid insecticides (so-called knockdown resistance or kdr). The L1014F (leucine-to-phenylalanine) mutation located in the centre of segment 6 of the domain 11 region (IIS6) of the sodium channel (the so-called kdr trait) has been detected in the peach-potato aphid, Myzus persicae (Sulzer), and is considered to be the primary cause of pyrethroid resistance in this species. Here we report on the characterisation of a second mutation, M918T (methione-to-threonine), within the nearby IIS4-S5 intracellular linker (the so-called super-kdr trait) in a field clone also possessing L1014F, with both mutations present in heterozygous form. The resistance phenotype of M. persicae clones possessing various combinations of L1014F and M918T to a wide range of pyrethroids (both Type I and II) was assessed in leaf-dip bioassays and to lambda-cyhalothrin applied at up to ten times the recommended field rate as foliar sprays to aphids feeding on whole plants. Bioassay results demonstrated that presence of both mutations was associated with extreme resistance to all the pyrethroids tested relative to aphids lacking the mutations. Furthermore, this resistance well exceeded that shown by aphids that were homozygous for L1014F but lacking M918T. However, pre-treatment with piperonyl butoxide in the leaf-dip bioassays failed to suppress pyrethroid resistance in aphids carrying one or both of the mutations. The relevance of these findings for monitoring and managing pyrethroid resistance in M. persicae populations in the field is discussed.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofBulletin of entomological research
dc.subjectKNOCKDOWN RESISTANCE
dc.subjectHOUSEFLY MUSCA-DOMESTICA
dc.subjectSIDE-CHAINS
dc.subjectMOLECULAR CHARACTERIZATION
dc.subjectpyrethroicis
dc.subjectALCOHOL COMPONENT
dc.subjectBLATTELLA-GERMANICA
dc.subjectknockdown resistance
dc.subjectHEMIPTERA
dc.subjectIDENTIFICATION
dc.subjectPOINT MUTATIONS
dc.subjectsuper-kdr mutation
dc.subjectsodium channel
dc.subjectMyzus persicae
dc.subjectSUPER-KDR
dc.titleCharacterization of the M918T sodium channel gene mutation associated with strong resistance to pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer)en
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2008-04
rioxxterms.versionofrecordhttps://doi.org/10.1017/S0007485307005524
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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