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dc.contributor.authorJorfi, Samireh
dc.contributor.authorInal, Jameel
dc.date.accessioned2018-07-31T14:30:39Z
dc.date.available2018-07-31T14:30:39Z
dc.date.issued2012-09-13
dc.identifier.citationJorfi , S & Inal , J 2012 , ' Coxsackie virus entry and spread in HeLa cells is aided by microvesicle release ' Microvesiculation and Disease, a Biochemical Society Focused Meeting , London , United Kingdom , 13/09/12 - 14/09/12 , .
dc.identifier.citationconference
dc.identifier.otherPURE: 13382485
dc.identifier.otherPURE UUID: 9f554900-e92d-4309-81c9-bb463e52e3ad
dc.identifier.urihttp://hdl.handle.net/2299/20317
dc.description.abstractMicrovesicles(MVs) released from plasma membrane expressing surface phosphatidylserine and ranging from 0.2-≤1 m in diameter are reported to carry various membrane proteins, lipids and cytoplasmic components characteristic of the parental cell (1). Coxsackievirus B (CVB), a member of the enterovirus family is the main cause of meningitis and encephalitis in infants which may result in neurodevelopmental defects. Calpains are calcium-dependant cysteine proteases that degrade cytoplasmic and cytoskeletal proteins. They regulate a variety of actin-dependant cellular processes such as microvesiculation. CVB1 requires calpain activation for both entry and virus replication. Here, we show that knocking down calpain, using approaches such as small interfering RNA (siRNA), culminates in reduction of MV release, as we showed before with another intracellular pathogen, the protozoan parasite, Trypanosoma cruzi (2). The reduction in MV release then abrogates CVB1 entry and spread in HeLa cells. The calpain inhibitor calpeptin also caused similar reduction in CVB1 entry and spread to healthy target cells. Together, our findings provide evidence that CVB1 infected HeLa cells enhance MV production, and these MVs aid the spread of infection. Furthermore, inhibition of MV release using siRNA results in inhibition of CVB1 entry and spread.en
dc.language.isoeng
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.titleCoxsackie virus entry and spread in HeLa cells is aided by microvesicle releaseen
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Accepted Version
rioxxterms.versionAM
rioxxterms.typeOther
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeopenAccess


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