dc.contributor.author | Kosgodage, Uchini S | |
dc.contributor.author | Matewele, Paul | |
dc.contributor.author | Awamaria, Brigitte | |
dc.contributor.author | Kraev, Igor | |
dc.contributor.author | Warde, Purva | |
dc.contributor.author | Mastroianni, Giulia | |
dc.contributor.author | Nunn, alistair | |
dc.contributor.author | Guy, Geoffrey | |
dc.contributor.author | Bell, Jimmy | |
dc.contributor.author | Inal, Jameel | |
dc.contributor.author | Lange, Sigrun | |
dc.date.accessioned | 2019-09-17T14:04:33Z | |
dc.date.available | 2019-09-17T14:04:33Z | |
dc.date.issued | 2019-09-10 | |
dc.identifier.citation | Kosgodage , U S , Matewele , P , Awamaria , B , Kraev , I , Warde , P , Mastroianni , G , Nunn , A , Guy , G , Bell , J , Inal , J & Lange , S 2019 , ' Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles ' , Frontiers Cellular and Infection Microbiology , vol. 9 , 324 , pp. 1-13 . https://doi.org/10.3389/fcimb.2019.00324 | |
dc.identifier.issn | 2235-2988 | |
dc.identifier.uri | http://hdl.handle.net/2299/21686 | |
dc.description.abstract | Membrane vesicles (MVs) released from bacteria participate in cell communication and host-pathogen interactions. Roles for MVs in antibiotic resistance are gaining increased attention and in this study we investigated if known anti-bacterial effects of cannabidiol (CBD), a phytocannabinoid from Cannabis sativa, could be in part attributed to effects on bacterial MV profile and MV release. We found that CBD is a strong inhibitor of MV release from Gram-negative bacteria (E. coli VCS257), while inhibitory effect on MV release from Gram-positive bacteria (S. aureus subsp. aureus Rosenbach) was negligible. When used in combination with selected antibiotics, CBD significantly increased the bactericidal action of several antibiotics in the Gram-negative bacteria. In addition, CBD increased antibiotic effects of kanamycin in the Gram-positive bacteria, without affecting MV release. CBD furthermore changed protein profiles of MVs released from E. coli after 1 h CBD treatment. Our findings indicate that CBD may pose as a putative adjuvant agent for tailored co-application with selected antibiotics, depending on bacterial species, to increase antibiotic activity, including via MV inhibition, and help reduce antibiotic resistance. | en |
dc.format.extent | 13 | |
dc.format.extent | 851355 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers Cellular and Infection Microbiology | |
dc.subject | E. coli VCS257 | |
dc.subject | S. aureus subsp. aureus Rosenbach | |
dc.subject | antibiotic resistance | |
dc.subject | bacterial membrane vesicles (MVs) | |
dc.subject | cannabidiol (CBD) | |
dc.subject | gram-negative | |
dc.subject | gram-positive | |
dc.subject | Microbiology | |
dc.subject | Immunology | |
dc.subject | Microbiology (medical) | |
dc.subject | Infectious Diseases | |
dc.title | Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles | en |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Extracellular Vesicle Research Unit | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85072623682&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.3389/fcimb.2019.00324 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |