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Browsing University of Hertfordshire by Author "Kukol, A."
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A cellulose-based bioassay for the colorimetric detection of pathogen DNA
Saikrishnan, Deepika; Goyal, Madhu; Rossiter, Sharon; Kukol, A. (2014-12-01)Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen ... -
Conformational Flexibility of the Peptide Hormone Ghrelin in Solution and Lipid Membrane Bound: A Molecular Dynamics Study
Beevers, A.J.; Kukol, A. (2006)Human ghrelin is a peptide hormone of 28 aminoacid residues, in which the Ser3 is modified by an octanoyl group. Ghrelin has a major role in the energy metabolism of the human body stimulating growth hormone release as ... -
Consensus virtual screening approaches to predict protein ligands
Kukol, A. (2011)In order to exploit the advantages of receptor-based virtual screening, namely time/cost saving and specificity, it is important to rely on algorithms that predict a high number of active ligands at the top ranks of a small ... -
Electrostatic determinants of the ion channel control of the nicotinic acetylcholine receptor of Torpedo californica
Kukol, A.; Neumann, E. (1998)The patch clamp K+-conductance G of the nicotinic acetylcholine receptor (AcChoR) dimer (M(r)approximate to 590 000) of Torpedo californica, reconstituted in lipid vesicles, icles, which decreases with increasing Cain the ... -
Enhancing resolution and sensitivity of 17O solid-state NMR through combining double rotation, 1H decoupling and satellite modulation for biomolecular applications
Howes, P.; Anupolde, T.; Lemaitre, V.; Kukol, A.; Watts, A.; Samosone, A.; Smith, M.E.; Dupree, R. (2006) -
Enzyme Kinetics Lecture Notes
Kukol, A. (Create Space Independent Publishing Platform, 2015-08-14)This book covers the topic of enzyme kinetics for a three-year undergraduate programme in bioscience. It begins with a thorough introduction into chemical kinetics, which forms the basis of all enzyme kinetics application. ... -
Experimentally based orientational refinement of membrane protein models : a structure for the Influenza A M2 H+ channel
Kukol, A.; Adams, P.D.; Rice, L.M.; Brunger, A.T.; Arkin, I.T. (1999)The structure determination of membrane proteins is still a difficult task. Considering the fact that as many as 30% or more of cellular proteins are associated with the membrane (Arkin et al., 1997a), the paucity of high ... -
Exploring models of the influenza A M2 channel: MD simulations in a phospholipid bilayer
Forrest, L. R.; Kukol, A.; Arkin, I.T.; Tieleman, D. P.; Sansom, M. S. P. (2000-01)The M2 protein of influenza A virus forms homotetrameric helix bundles, which function as proton-selective channels. The native form of the protein is 97 residues long, although peptides representing the transmembrane ... -
G-quadruplex formation of FXYD1 pre-mRNA indicates the possiblity of regulating expression of its protein product
Dhayan, Hansraj; Baydoun, A. R.; Kukol, A. (2014-10-15)G-quadruplexes are higher-order nucleic acid structures formed of square-planar arrangements of four guanine bases held together by Hoogsteen-type hydrogen bonds. Stacks of guanine tetrads are stabilised by intercalating ... -
How the amyloid-β peptide and membranes affect each other : An extensive simulation study
Poojari, Chetan; Kukol, A.; Strodel, Birgit (2013-02)The etiology of Alzheimer's disease is thought to be linked to interactions between amyloid-β (Aβ) and neural cell membranes, causing membrane disruption and increased ion conductance. The effects of Aβ on lipid behavior ... -
Incorporation of the acetylcholine receptor dimer from Torpedo californica in a peptide supported lipid membrane investigated by surface plasmon and fluorescence spectroscopy
Schmidt, E. K.; Liebermann, T.; Kreiter, M.; Jonczyk, A.; Naumann, R.; Offenhausser, A.; Neumann, E.; Kukol, A.; Maelicke, A.; Knoll, W. (1998-09-15)The dimer species (M-r 580 000) of the nicotinic acetylcholine receptor, isolated from the electric organ of Torpedo californica, was incorporated into a thiopeptide supported lipid bilayer. The incorporation was achieved ... -
Influence of divalent cations on the conductance of the torpedo-acetylcholine receptor.
Kukol, A.; Neumann, E. (1996) -
Influenza A nucleoprotein binding sites for antivirals: : current research and future potential
Kukol, A.; Patel, Hershna (2014-09-15) -
Ion channels - Analysis and simulation embedded in a flexible environment
Kukol, A. (1998-03-25)A collection of programs for ion-channel analysis is described. The package contains all standard routines for single-channel analysis and some routines for fluctuation analysis. Additionally, advanced algorithms for ... -
Label free electrical detection of DNA hybridisation for the example of influenza virus gene sequences
Kukol, A.; Li, P.; Estrela, P.; Ko-Ferrigno, P.; Migliorato, P. (2008)Microarrays based on DNA–DNA hybridization are potentially useful for detecting and subtyping viruses but require fluorescence labeling and imaging equipment. We investigated a label-free electrical detection system using ... -
Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites
Kukol, A.; Hughes, David (2014-04-30)The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus ... -
Large-scale analysis of Influenza A virus sequences reveals potential drug-target sites of non-structural proteins
Darapaneni, V.; Prabhakar, V.; Kukol, A. (2009-10)The non-structural protein 1 (NS1) of the influenza A virus and the NS2 protein, which is also known as nuclear export protein, play important roles in the infectious life cycle of the virus. The objective of this study ... -
Lipid membranes for membrane proteins
Kukol, A. (Springer Nature, 2015-01-01) -
Lipid models for united-atom molecular dynamics simulations of proteins
Kukol, A. (2009)United-atom force fields for molecular dynamics (MD) simulations provide a higher computational efficiency, especially in lipid membrane simulations, with little sacrifice in accuracy, when compared to all-atom force fields. ... -
Mapping the energy surface of transmembrane helix-helix interactions
Torres, J.; Kukol, A.; Arkin, I.T. (2001-11)Transmembrane helices are no longer believed to be just hydrophobic segments that exist solely to anchor proteins to a lipid bilayer, but rather they appear to have the capacity to specify function and structure. Specific ...