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On the relationship between nucleotide hydrolysis and microtubule assembly : Studies with a GTP-regenerating system
The assembly of pure tubulin dimer has been studied in two buffer systems (containing low and high glycerol/Mg), using a regeneration system protocol to assess the amount of GDP-tubulin in the assembling polymer. For both ...
Dynamic properties of microtubules at steady state of polymerisation
Microtubules of tubulin dimer, polymerised in vitro to steady-state are shown to incorporate tubulin rapidly and extensively. The method involves adding [3H]-GTP to microtubules at steady state, and analysing for ...