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        Characterisation of the Interaction of Lactate Dehydrogenase With Tween-20 Using Isothermal Titration Calorimetry, Interfacial Rheometry and Surface Tension Measurements

        Author
        Mcauley, William J.
        Jones, David S.
        Kett, Vicky L.
        Attention
        2299/9587
        Abstract
        In this study the nature of the interaction between Tween-20 and lactate dehydrogenase (LDH) was investigated using isothermal titration calorimetry (ITC). In addition the effects of the protein and surfactant on the interfacial properties were followed with interfacial rheology and surface tension measurements in order to understand the mechanism by which the surfactant prevents protein adsorption to the air-water interface. Comparisons were made with Tween-40 and Tween-80 in order to further investigate the mechanism. ITC measurements indicated a weak, probably hydrophobic, interaction between Tween-20 and LDH. Prevention of LDH adsorption to the air-water interface by the Tween surfactants was correlated with Surface energy rather than surfactant CMC. While surface pressure appears to be the main driving force for the displacement of LDH from the air-water interface by Tween-20 a solubilisation mechanism may exist for other protein molecules. More generally the results of this study highlight the value of the use of ITC and interfacial measurements in characterising the surface behaviour of mixed surfactant and protein systems. (C) 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:2659-2669, 2009
        Publication date
        2009-08
        Published in
        Journal of Pharmaceutical Sciences
        Published version
        https://doi.org/10.1002/jps.21640
        Other links
        http://hdl.handle.net/2299/9587
        Relations
        School of Life and Medical Sciences
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