dc.contributor.author | Mkrtchyan, Hermine | |
dc.contributor.author | Gibbons, Simon | |
dc.contributor.author | Heidelberger, Sibylle | |
dc.contributor.author | Zloh, Mire | |
dc.contributor.author | Limaki, Hamidreza Khalatbari | |
dc.date.accessioned | 2013-04-25T09:40:00Z | |
dc.date.available | 2013-04-25T09:40:00Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Mkrtchyan , H , Gibbons , S , Heidelberger , S , Zloh , M & Limaki , H K 2010 , ' Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narine ' , International Journal of Antimicrobial Agents , vol. 35 , no. 3 , pp. 255-60 . https://doi.org/10.1016/j.ijantimicag.2009.11.017 | |
dc.identifier.other | PURE: 1457556 | |
dc.identifier.other | PURE UUID: 8ac5ac6d-38b4-4239-b749-1243176be360 | |
dc.identifier.other | PubMed: 20045288 | |
dc.identifier.other | Scopus: 73549120400 | |
dc.identifier.uri | http://hdl.handle.net/2299/10562 | |
dc.description | Copyright 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. | |
dc.description.abstract | In the last two decades, antimicrobial peptides (AMPs) have been gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Lactobacillus acidophilus n.v. Er 317/402 strain Narine produces a small AMP with a molecular weight of 1.1kDa, designated acidocin LCHV. In this study, the AMP was extremely heat stable (90min at 130 degrees C), was active over a wide pH range and was found to be sensitive to proteolytic enzymes (trypsin, pepsin and proteinase K). Acidocin LCHV has a broad spectrum of activity both against Gram-positive and Gram-negative pathogens, including several that are classified as Especially Dangerous Infections by the World Health Organization as well as meticillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) was used to determine the molecular mass and sequence of the purified peptide. Complete killing with immediate impact on cells was observed within a very short period of time (10min). | en |
dc.format.extent | 6 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Antimicrobial Agents | |
dc.title | Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narine | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Department of Pharmacy | |
dc.contributor.institution | Medicinal and Analytical Chemistry | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | https://doi.org/10.1016/j.ijantimicag.2009.11.017 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |