dc.contributor.author | Chau, David Y.S. | |
dc.contributor.author | Tint, Naing L. | |
dc.contributor.author | Collighan, Russell J. | |
dc.contributor.author | Griffin, Martin | |
dc.contributor.author | Dua, Harminder S. | |
dc.contributor.author | Shakesheff, Kevin M. | |
dc.contributor.author | Rose, Felicity R. A. J. | |
dc.date.accessioned | 2013-12-03T16:00:01Z | |
dc.date.available | 2013-12-03T16:00:01Z | |
dc.date.issued | 2010-05 | |
dc.identifier.citation | Chau , D Y S , Tint , N L , Collighan , R J , Griffin , M , Dua , H S , Shakesheff , K M & Rose , F R A J 2010 , ' The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles ' , British Journal of Ophthalmology , vol. 94 , no. 5 , pp. 648-653 . https://doi.org/10.1136/bjo.2009.163642 | |
dc.identifier.issn | 0007-1161 | |
dc.identifier.uri | http://hdl.handle.net/2299/12257 | |
dc.description | This paper is freely available online under the BMJ Journals unlocked scheme, see http:// bjo.bmj.com/site/about/unlocked.xhtm | |
dc.description.abstract | Aims To demonstrate the potential use of in vitro poly (lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. Methods PLGA microparticles (diameter 10-60 mu m) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. Results Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. Conclusion The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension. | en |
dc.format.extent | 6 | |
dc.format.extent | 998498 | |
dc.language.iso | eng | |
dc.relation.ispartof | British Journal of Ophthalmology | |
dc.subject | PREFERRED SUBSTRATE SEQUENCES | |
dc.subject | DISPLAYED PEPTIDE LIBRARY | |
dc.subject | TRIAMCINOLONE ACETONIDE | |
dc.subject | ANTERIOR-CHAMBER | |
dc.subject | MICROBIAL TRANSGLUTAMINASE | |
dc.subject | TISSUE TRANSGLUTAMINASE | |
dc.subject | CATARACT-SURGERY | |
dc.subject | MICROSPHERES | |
dc.subject | CAPSULE | |
dc.subject | CELLS | |
dc.title | The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Department of Pharmacy | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1136/bjo.2009.163642 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |