dc.contributor.author | Antwi-Baffour, Samuel | |
dc.contributor.author | Malibha-Pinchbeck, Memory | |
dc.contributor.author | Stratton, Dan | |
dc.contributor.author | Jorfi, Samireh | |
dc.contributor.author | Lange, Sigrun | |
dc.contributor.author | Inal, Jameel | |
dc.date.accessioned | 2019-12-24T01:05:27Z | |
dc.date.available | 2019-12-24T01:05:27Z | |
dc.date.issued | 2019-12-18 | |
dc.identifier.citation | Antwi-Baffour , S , Malibha-Pinchbeck , M , Stratton , D , Jorfi , S , Lange , S & Inal , J 2019 , ' Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets ' , Journal of Extracellular Vesicles , vol. 9 , no. 1 , 1697124 , pp. 1-13 . https://doi.org/10.1080/20013078.2019.1697124 | |
dc.identifier.uri | http://hdl.handle.net/2299/22031 | |
dc.description | © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.description.abstract | This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, (ii) period from onset of malaria symptoms until seeking medical care (patient delay, PD), (iii) age and (iv) gender. In this cross-sectional study, n = 434 patients were analysed and Nanosight Tracking Analysis (NTA) used to quantify mEVs (vesicles of 150–500 nm diameter, isolated at 15,000 × g, β-tubulin-positive and staining for annexin V, but weak or negative for CD81). Overall plasma mEV levels (1.69 × 10 10 mEVs mL −1) were 2.3-fold higher than for uninfected controls (0.51 × 10 10 mEVs mL −1). Divided into four age groups, we found a bimodal distribution with 2.5- and 2.1-fold higher mEVs in infected children (<11 years old [yo]) (median:2.11 × 10 10 mEVs mL −1) and the elderly (>45 yo) (median:1.92 × 10 10 mEVs mL −1), respectively, compared to uninfected controls; parasite density varied similarly with age groups. There was a positive association between mEVs and parasite density (r = 0.587, p < 0.0001) and mEVs were strongly associated with PD (r = 0.919, p < 0.0001), but gender had no effect on plasma mEV levels (p = 0.667). Parasite density was also exponentially related to patient delay. Gender (p = 0.667) had no effect on plasma mEV levels. During periods of low parasitaemia (PD = 72h), mEVs were 0.93-fold greater than in uninfected controls. As 75% (49/65) of patients had low parasitaemia levels (20–500 parasites µL −1), close to the detection limits of microscopy of Giemsa-stained thick blood films (5–150 parasites µL −1), mEV quantification by NTA could potentially have early diagnostic value, and raises the potential of Pf markers in mEVs as early diagnostic targets. | en |
dc.format.extent | 13 | |
dc.format.extent | 2541928 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Extracellular Vesicles | |
dc.subject | Malaria | |
dc.subject | extracellular vesicles | |
dc.subject | parasitaemia | |
dc.subject | Histology | |
dc.subject | Cell Biology | |
dc.title | Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets | en |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Extracellular Vesicle Research Unit | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85076903602&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1080/20013078.2019.1697124 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |