dc.contributor.author | Hassan, Sidra | |
dc.contributor.author | Syun-Ichi, Urayama | |
dc.contributor.author | Shabeer, Saba | |
dc.contributor.author | Wu, Chien-Fu | |
dc.contributor.author | Moriyama, Hiromitsu | |
dc.contributor.author | Coutts, Robert H A | |
dc.contributor.author | Kotta-Loizou, Ioly | |
dc.contributor.author | Jamal, Atif | |
dc.date.accessioned | 2024-01-09T10:30:01Z | |
dc.date.available | 2024-01-09T10:30:01Z | |
dc.date.issued | 2023-11-28 | |
dc.identifier.citation | Hassan , S , Syun-Ichi , U , Shabeer , S , Wu , C-F , Moriyama , H , Coutts , R H A , Kotta-Loizou , I & Jamal , A 2023 , ' Molecular and biological characterization of a partitivirus from Paecilomyces variotii. ' , Journal of General Virology , vol. 104 , no. 11 , 001925 , pp. 1-13 . https://doi.org/10.1099/jgv.0.001925 | |
dc.identifier.issn | 0022-1317 | |
dc.identifier.other | ORCID: /0000-0003-3277-6359/work/150595208 | |
dc.identifier.uri | http://hdl.handle.net/2299/27382 | |
dc.description | © 2023 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/ | |
dc.description.abstract | Paeciliomyces variotii is a thermo-tolerant, ubiquitous fungus commonly found in food products, indoor environments, soil and clinical samples. It is a well-known biocontrol agent used against phytopathogenic fungi and its metabolites have many industrial applications. Rare reports of P. variotii-related human infections have been found in the medical literature. In this study, we report for the first time the infection of P. variotii isolated from a soil sample collected in a rice field with a double-stranded RNA virus, Paeciliomyces variotii partitivirus 1 (PvPV-1) in the family Partitiviridae. P. variotii harboured icosahedral virus particles 30 nm in diameter with two dsRNA segments 1758 and 1356 bp long. Both dsRNA1 and dsRNA2 have a single open reading frame encoding proteins of 63 and 40 kDa, respectively. These proteins have significant similarity to the RNA-dependent RNA polymerase and capsid protein encoded by the genomic segments of several viruses from the family Partitiviridae. Phylogenetic analysis revealed that PvPV-1 belongs to the family Partitiviridae but in an unclassified group/genus, tentatively nominated Zetapartitivirus. PvPV-1 was found to increase the growth rate of the host fungus, as indicated by time course experiments performed on a range of different media for virus-infected and virus-free isogenic lines. Further, dual-culture assays performed for both isogenic lines confirmed the antagonistic potential of P. variotii against other phytopathogenic fungi. The findings of this study assist us in understanding P. variotii as a potential biocontrol agent, together with plant-fungus-virus interactions. | en |
dc.format.extent | 13 | |
dc.format.extent | 8601584 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of General Virology | |
dc.subject | Humans | |
dc.subject | Phylogeny | |
dc.subject | Byssochlamys | |
dc.subject | Capsid Proteins | |
dc.subject | Soil | |
dc.subject | Partitiviridae | |
dc.subject | zetapartitivirus | |
dc.subject | Paecilomyces variotii | |
dc.subject | biological control | |
dc.subject | growth | |
dc.subject | metabolism | |
dc.subject | double-stranded RNA | |
dc.subject | Virology | |
dc.title | Molecular and biological characterization of a partitivirus from Paecilomyces variotii. | en |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Agriculture, Food and Environmental Management Research | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | University of Hertfordshire | |
dc.contributor.institution | Department of Clinical and Pharmaceutical Sciences | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85178497153&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1099/jgv.0.001925 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |