Aspects of Growth and Metabolism in a Suspension Culture of Acer pseudoplatanus (L. ) Grown on a Glycerol Carbon Source
Grout, B.W.W.; Chan, K.W.; Simpkins, I.
Citation: Grout , B W W , Chan , K W & Simpkins , I 1976 , ' Aspects of Growth and Metabolism in a Suspension Culture of Acer pseudoplatanus (L. ) Grown on a Glycerol Carbon Source ' Journal of Experimental Botany , vol 27 , no. 1 , pp. 77-84 . DOI: 10.1093/jxb/27.1.77
A suspension culture of Acer pseudoplatanus cells was transferred from medium containing 2% (w/v) glucose to an identical one containing glycerol at 2% (w/v) as the sole carbon source. The patterns of cell number increase, dry weight increase, and changes in packed cell volume showed marked differences as a result of this transfer. The glucose-grown cultures contained a small proportion of cells of exceptionally large diameter, and transfer to the glycerol carbon source appeared to bring about a considerable increase in their number. These larger cells, in both glucose- and glycerol-containing cultures, exhibited considerable differences in cell wall architecture when compared with their smaller counterparts. They appeared more irregular, and had much looser cellulose microfibril arrangement in their outer layers
Original article can be found at: http://jxb.oxfordjournals.org/ Copyright Society for Experimental Biology [Full text of this article is not available in the UHRA]
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