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Browsing by Author "Adams, Michael J."
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Characterization of Polymyxa species by restriction analysis of PCR-amplified ribosomal DNA
Ward, Elaine; Adams, Michael J.; Mutasa, E. S.; Collier, C. R.; Asher, M. J. C. (1994-10)A molecular method is described to aid identification of the obligate parasite Polymyxa and discriminate between species (P. betae and P. graminis) and isolates. DNA was extracted from zoospores, resting spores and roots ... -
Development of PCR for the detection of Polymyxa-betae in sugar beet roots and its application in field studies
Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Adams, Michael J.; Ward, Elaine; Asher, M. J. C. (1995-11)A previously cloned 1.8 kb PCR Polymyxa betae DNA fragment has been fully sequenced and used to design PCR primers for the specific detection of P. betae in sugar beet seedling roots. The primers did not amplify sequences ... -
Evidence that Polymyxa species may infect Arabidopsis thaliana
Smith, Madeleine J.; Adams, Michael J.; Ward, Elaine (2011-05)Polymyxa spp. are obligate biotrophs belonging to the plasmodiophorid group, responsible for transmitting a large number of plant viruses to many crop species. Their obligate nature makes them difficult to study. Controlled ... -
The use of conventional and quantitative real-time PCR assays for Polymyxa graminis to examine host plant resistance, inoculum levels and intraspecific variation
Ward, Elaine; Kanyuka, K.; Motteram, J.; Kornyukhin, D.; Adams, Michael J. (2005-03)A real-time PCR protocol based on 18S rDNA sequences was developed to provide a specific, sensitive and quantitative assay for the root-infecting virus vector Polymyxa graminis. The assay was calibrated with zoospore ...