Browsing by Author "Brocchini, Steve"

Now showing items 1-12 of 12

    • Disulfide bridge based PEGylation of proteins 

      Brocchini, Steve; Godwin, Antony; Balan, Sibu; Choi, Ji-won; Zloh, Mire; Shaunak, Sunil (2008-01)
      PEGylation is a clinically proven strategy for increasing the therapeutic efficacy of protein-based medicines. Our approach to site-specific PEGylation exploits the thiol selective chemistry of the two cysteine sulfur atoms ...

    • From sequence to 3D structure of hyperbranched molecules : application to surface modified PAMAM dendrimers 

      Barata, Teresa S.; Brocchini, Steve; Teo, Ian; Shaunak, Sunil; Zloh, Mire (2011)
      The molecular modeling of hyperbranched molecules is currently constrained by difficulties in model building, due partly to lack of parameterization of their building blocks. We have addressed this problem with specific ...

    • Identification of excipient-protein interaction hotspots using computational approaches 

      Barata, Teresa S; Zhang, Cheng; A. Darby, Paul; Brocchini, Steve; Zloh, Mire (2016-06-01)
      Protein formulation development relies on the selection of excipients that inhibit protein-protein interactions preventing aggregation. Empirical strategies involve screening many excipient and buffer combinations using ...

    • Molecular modeling to study dendrimers for biomedical applications 

      Martinho, Nuno; Florindo, Helena; Silva, Liana; Brocchini, Steve; Zloh, Mire; Barata, Teresa (2014-12-08)
      Molecular modeling techniques provide a powerful tool to study the properties of molecules and their interactions at the molecular level. The use of computational techniques to predict interaction patterns and molecular ...

    • Optimization of protein loaded PLGA nanoparticles manufacturing parameters following a quality-by-design approach 

      Sainz, Vanessa; Peres, Carina; Ciman, Tamara; Rodrigues, Catarina; Viana, Ana; Alfonso, Carlo A. M.; Barata, Teresa S; Brocchini, Steve; Zloh, Mire; Gaspar, Rogerio S.; Florindo, Helena; Lopes, Joao (2016-10-26)
      Development of a multivariate-based regression model for estimating the critical attributes for establishing a design-space for poly(lactic-co-glycolic acid) (PLGA) nanoparticles formulated by a double emulsion-solvent ...

    • PEGylation of native disulfide bonds in proteins 

      Brocchini, Steve; Balan, Sibu; Godwin, Antony; Choi, Ji-Won; Zloh, Mire; Shaunak, Sunil (2006)
      PEGylation has turned proteins into important new biopharmaceuticals. The fundamental problems with the existing approaches to PEGylation are inefficient conjugation and the formation of heterogeneous mixtures. This is ...

    • Poly-glutamic dendrimer-based conjugates for cancer vaccination - a computational design for targeted delivery of antigens 

      Moura, Liane; Martinho, Nuno; Silva, Liana; Barata, Teresa S; Brocchini, Steve; Florindo, Helena; Zloh, Mire (2017-08-10)
      Computational techniques are useful to predict interaction models and molecular properties for the design of drug delivery systems, such as dendrimers. Dendrimers are hyperbranched macromolecules with repetitive building ...

    • Practical computational toolkits for dendrimers and dendrons structure design 

      Martinho, Nuno; Silva, Liana; Florindo, Helena; Brocchini, Steve; Barata, Teresa S; Zloh, Mire (2017-09-30)
      Dendrimers and dendrons offer an excellent platform for developing novel drug delivery systems and medicines. The rational design and further development of these repetitively branched systems are restricted by difficulties ...

    • Rational design of novel fluorescent tagged glutamic acid dendrimers with different terminal groups and in-silico analysis of their properties 

      Martinho, Nuno; Silva, Liana; Florindo, Helena; Brocchini, Steve; Zloh, Mire; Barata, Teresa S (2017-09-25)
      Dendrimers are hyperbranched polymers with a multifunctional architecture that can be tailored for the use in various biomedical applications. Peptide dendrimers are particularly relevant for drug delivery applications due ...

    • Site-specific PEGylation at histidine tags 

      Cong, Yuehua; Pawlisz, Estera; Bryant, Penny; Balan, Sibu; Laurine, Emmanuelle; Tommasi, Rita; Singh, Ruchi; Dubey, Sitara; Peciak, Karolina; Bird, Matthew; Sivasankar, Amrita; Swierkosz, Julia; Muroni, Maurizio; Heidelberger, Sibylle; Farys, Monika; Khayrzad, Farzad; Edwards, Jeff; Badescu, George; Hodgson, Ian; Heise, Charles; Somavarapu, Satyanarayana; Liddell, John; Powell, Keith; Zloh, Mire; Choi, Ji-won; Godwin, Antony; Brocchini, Steve (2012)
      The efficacy of protein-based medicines can be compromised by their rapid clearance from the blood circulatory system. Achieving optimal pharmacokinetics is a key requirement for the successful development of safe protein-based ...

    • Site-specific PEGylation of native disulfide bonds in therapeutic proteins 

      Shaunak, Sunil; Godwin, Antony; Choi, Ji-Won; Balan, Sibu; Pedone, Elisa; Vijayarangam, Damotharan; Heidelberger, Sibylle; Teo, Ian; Zloh, Mire; Brocchini, Steve (2006)
      Native disulfide bonds in therapeutic proteins are crucial for tertiary structure and biological activity and are therefore considered unsuitable for chemical modification. We show that native disulfides in human interferon ...

    • Structural Studies on Glycosylated PAMAM Dendrimers 

      Barata, Teresa S.; Brocchini, Steve; Teo, Ian; Shaunak, Sunil; Zloh, Mire (2010-10)