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Browsing by Author "Coffin, R.S."
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Beet pseudo-yellows virus is an authentic closterovirus
Coutts, Robert H.A.; Coffin, R.S. (1996-01)We have previously used RT-PCR to amplify a 251 base pair fragment diagnostic for beet pseudo-yellows virus (BPYV) infection from several BPYV infected samples from Europe and the USA. This has now been shown by sequencing ... -
The closteroviruses, capilloviruses and other similar viruses : A short review
Coffin, R.S.; Coutts, Robert H.A. (1993-08)The closteroviruses and capilloviruses are diverse groups of structurally similar, flexuous, filamentous plant viruses. However, because of a lack of information of other than a morphological nature the taxonomy of these ... -
DsRNA cloning and diagnosis of beet pseudo-yellows virus by PCR and nucleic acid hybridization
Coffin, R.S.; Coutts, Robert H.A. (1992)DsRNA has been extracted from beet pseudo-yellows virus infected cucumber plants, purified to homogeneity, and cDNA clones to it produced. The clones are of insufficient sensitivity to detect infection-specific RNA in dot ... -
The infectivity of dimeric potato yellow mosaic geminivirus clones in different hosts
Buragohain, A.K.; Sung, Y.K.; Coffin, R.S.; Coutts, Robert H.A. (1994-10)Head-to-tail dimeric clones of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV) were constructed. These constructs were infectious when inoculated onto Nicotiana benthamiana plants either as DNA or by ... -
The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus
Coutts, Robert H.A.; Coffin, R.S.; Roberts, E.J.F.; Hamilton, W.D.O. (1991-07)The complete nucleotide sequence of a Venezuelan isolate of potato yellow mosaic virus (PYMV) has been determined, showing it to be typical of subgroup I geminiviruses in that it is whitefly-transmitted, has a circular, ... -
The tobacco necrosis virus p7a protein is a nucleic acid-binding protein
Offei, S.K.; Coffin, R.S.; Coutts, Robert H.A. (1995-06)The two centrally located open reading frames (ORFs) of necroviruses may, by analogy with the similarly located and related ORFs of carmoviruses, be expected to have a function in virus movement in plants. In the case of ...