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Browsing by Author "Good, Liam"
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Antisense PNA accumulates in Escherichia coli and mediates a long post-antibiotic effect
Nikravesh, Abbas; Dryselius, Rikard; Faridani, Omid R; Goh, Shan; Sadeghizadeh, Majid; Behmanesh, Mehrdad; Ganyu, Anita; Klok, Erik Jan; Zain, Rula; Good, Liam (2007-08-01)Antisense agents that target growth-essential genes display surprisingly potent bactericidal properties. In particular, peptide nucleic acid (PNA) and phosphorodiamidate morpholino oligomers linked to cationic carrier ... -
Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli
Goh, Shan; Boberek, Jaroslaw M; Nakashima, Nobutaka; Stach, Jem; Good, Liam (2009-06-26)BACKGROUND: Genes essential for bacterial growth are of particular scientific interest. Many putative essential genes have been identified or predicted in several species, however, little is known about gene expression ... -
The development of species-specific antisense peptide nucleic acid method for the treatment and detection of viable Salmonella
Adebowale, Oluwawemimo; Goh, Shan; Good, Liam (2020-06)Genotypic based detection methods using specific target sites in the pathogen genome can complement phenotypic identification. We report the development of species-specific antisense peptide nucleic acid (PNA) combined ... -
Oxacillin sensitization of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius by antisense peptide nucleic acids in vitro
Goh, Shan; Loeffler, Anette; Lloyd, David H; Nair, Sean P; Good, Liam (2015-11-11)BACKGROUND: Antibiotic resistance genes can be targeted by antisense agents, which can reduce their expression and thus restore cellular susceptibility to existing antibiotics. Antisense inhibitors can be gene and pathogen ... -
Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli
Goh, Shan; Hohmeier, Angela; Stone, Timothy C; Offord, Victoria; Sarabia, Francisco; Garcia-Ruiz, Cristina; Good, Liam (2015-08-15)Essential bacterial genes located within operons are particularly challenging to study independently because of coordinated gene expression and the nonviability of knockout mutants. Essentiality scores for many operon genes ... -
Species-selective killing of bacteria by antimicrobial peptide-PNAs
Mondhe, Madhav; Chessher, Ashley; Goh, Shan; Good, Liam; Stach, James E M (2014-02-18)Broad-spectrum antimicrobials kill indiscriminately, a property that can lead to negative clinical consequences and an increase in the incidence of resistance. Species-specific antimicrobials that could selectively kill ... -
Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
Uguru, Gabriel C; Mondhe, Madhav; Goh, Shan; Hesketh, Andrew; Bibb, Mervyn J; Good, Liam; Stach, James E M (2013-06-27)We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic ...