- UHRA Home
- Browsing by Author
Browsing by Author "Witchell, J."
Now showing items 1-4 of 4
-
Cytokine Gene and Protein Expression in BCG Vaccinated and Non-Vaccinated Mycobacterium bovis Infected cattle
Witchell, J. (2009-07-01)The persistent increase of bovine tuberculosis (bTB) over the past twenty years has put a substantial strain on both the British economy and the welfare of livestock. However, the development of an effective bTB vaccine ... -
Cytokine mRNA expression in cattle infected with different dosages of Mycobacterium bovis
Boddu-Jasmine, H.C.; Witchell, J.; Vordermeier, M.; Wangoo, A.; Goyal, M. (2008)Cytokine mRNA expression of pro-inflammatory cytokines, i.e., interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and anti-inflammatory cytokines, i.e., interleukin-4 (IL-4), interleukin-10 (IL-10) was quantified ... -
RNA isolation and quantitative PCR from HOPE- and formalin-fixed bovine lymph node tissues
Witchell, J.; Varshney, D.; Gajjar, T.; Wangoo, A.; Goyal, M. (2008)The use of RNA extracted from HOPE fixed tissues in quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is fairly novel. We compared qRT-PCR analysis of formalin and HOPE fixed, paraffin embedded lymph ... -
Time dependent expression of cytokines in Mycobacterium bovis infected cattle lymph nodes
Witchell, J.; Maddipatia, S.V.P.K.; Wangoo, A.; Vordermeier, M.; Goyal, M. (2010)Advancements in the current diagnostic and vaccination protocols employed against bovine tuberculosis rely heavily upon a sound knowledge of the bovine immunological response. Central to this is the importance of timing ...