dc.contributor.author | Freeman, J. | |
dc.contributor.author | Ward, Elaine | |
dc.contributor.author | Gutteridge, R.J. | |
dc.contributor.author | Bateman, Geoffrey L. | |
dc.date.accessioned | 2013-04-09T07:59:43Z | |
dc.date.available | 2013-04-09T07:59:43Z | |
dc.date.issued | 2005-10 | |
dc.identifier.citation | Freeman , J , Ward , E , Gutteridge , R J & Bateman , G L 2005 , ' Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici ' , Plant Pathology , vol. 54 , no. 5 , pp. 686-698 . https://doi.org/10.1111/j.1365-3059.2005.01252.x | |
dc.identifier.issn | 0032-0862 | |
dc.identifier.uri | http://hdl.handle.net/2299/10335 | |
dc.description.abstract | Field isolates (n = 144) of the wheat take-all fungus Gaeumannomyces graminis var. tritici (Ggt) were tested for sensitivity to silthiofam, a take-all-specific fungicide used as a seed treatment, and identified as A- or B-type by PCR-RFLP analysis of nuclear rDNA. A possible association was identified between polymorphisms in ITS2 of the nuclear rDNA and sensitivity to silthiofam. A Ggt-specific PCR assay was developed which simultaneously identified isolates of Ggt as A- or B-type, based on the polymorphisms in the nuclear rDNA. A highly significant correlation between Ggt type using the PCR assay and sensitivity to silthiofam was demonstrated in a collection of 358 isolates from three field experiments designed to test the effects of seed-treatment fungicides on take-all and Ggt populations in winter wheat. In one experiment the percentages of silthiofam-sensitive and B-type isolates were significantly less in populations from plots sown with silthiofam-treated seed in two consecutive years than in populations from plots sown with nontreated seed. However, silthiofam still provided a significant amount of control of take-all. The natural occurrence of fungicide-insensitive isolates, up to about 30% in soils in which the fungicide had never been used, is unusual. The new PCR assay provides a useful tool for studying the population structure of Ggt, and may provide a novel method for assessing the incidence of insensitivity to silthiofam (the target site for which has not yet been identified) in field populations of Ggt. | en |
dc.format.extent | 13 | |
dc.language.iso | eng | |
dc.relation.ispartof | Plant Pathology | |
dc.title | Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1111/j.1365-3059.2005.01252.x | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |