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dc.contributor.authorWard, Elaine
dc.contributor.authorKanyuka, K.
dc.contributor.authorMotteram, J.
dc.contributor.authorKornyukhin, D.
dc.contributor.authorAdams, Michael J.
dc.date.accessioned2013-04-09T08:29:38Z
dc.date.available2013-04-09T08:29:38Z
dc.date.issued2005-03
dc.identifier.citationWard , E , Kanyuka , K , Motteram , J , Kornyukhin , D & Adams , M J 2005 , ' The use of conventional and quantitative real-time PCR assays for Polymyxa graminis to examine host plant resistance, inoculum levels and intraspecific variation ' , New Phytologist , vol. 165 , no. 3 , pp. 875-885 . https://doi.org/10.1111/j.1469-8137.2004.01291.x
dc.identifier.issn0028-646X
dc.identifier.otherPURE: 1269956
dc.identifier.otherPURE UUID: b04eb6f5-a3c9-4fb6-98fb-fade30e59bb2
dc.identifier.otherWOS: 000227229800024
dc.identifier.otherScopus: 14244261837
dc.identifier.urihttp://hdl.handle.net/2299/10338
dc.description.abstractA real-time PCR protocol based on 18S rDNA sequences was developed to provide a specific, sensitive and quantitative assay for the root-infecting virus vector Polymyxa graminis. The assay was calibrated with zoospore suspensions and inoculated roots and then shown to work with naturally infected plant roots and infested soil. Both the temperate P. graminis ribotypes previously described are detected but are not distinguished. DNA from related plasmodiophorids and from a range of fungi and plants was not detected. Different genotypes of Triticum were grown in a soil infested with P. graminis and Soil-borne cereal mosaic virus (SBCMV). The genotypes differed in susceptibility to P. graminis, the least susceptible being the Triticum monococcum accession K-58505. Conventional PCR assays and sequencing of amplified rDNA fragments showed that P. graminis isolates infecting wheat were mostly, but not exclusively, of ribotype II. Ribotype II was clearly associated with SBCMV transmission and seems to occur preferentially on wheat whereas ribotype I is mostly associated with barley.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofNew Phytologist
dc.titleThe use of conventional and quantitative real-time PCR assays for Polymyxa graminis to examine host plant resistance, inoculum levels and intraspecific variationen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1111/j.1469-8137.2004.01291.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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