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dc.contributor.authorLiu, S.
dc.contributor.authorWang, H.
dc.contributor.authorZhang, Junhua
dc.contributor.authorFitt, Bruce D.L.
dc.contributor.authorXu, Z.
dc.contributor.authorEvans, Neal
dc.contributor.authorLiu, Y.
dc.contributor.authorYang, W.
dc.contributor.authorGuo, X. X.
dc.identifier.citationLiu , S , Wang , H , Zhang , J , Fitt , B D L , Xu , Z , Evans , N , Liu , Y , Yang , W & Guo , X X 2005 , ' In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorum ' , Plant Cell Reports , vol. 24 , no. 3 , pp. 133-144 .
dc.identifier.otherPURE: 1389067
dc.identifier.otherPURE UUID: 0e0bded5-94f9-4f4e-b9bf-5167c24be6a2
dc.identifier.otherScopus: 21144445138
dc.description.abstractThis paper describes a new protocol to develop doubled-haploid (DH) Brassica napus lines with improved resistance to Sclerotinia sclerotiorum. In this protocol, haploid seedlings derived from microspore cultures of B. napus were used to produce haploid calli for in vitro mutation-selection. For routine screening, mutation was induced by EMS (ethylmethane sulfonate) or occurred spontaneously, and screening for resistant mutants occurred on media with added oxalic acid (OA) as a selection agent. In tests with selected lines, the optimal concentration of EMS for mutation was determined to be 0.15%, and the optimal concentration of OA for in vitro screening was 3 mmol/l (half lethal dose was 3.1 mmol/l) for the first cycle of screening. There was an accumulated effect of OA toxicity on calli over two cycles of screening, but the growth and capacity of the surviving calli for regenerating seedlings were not affected by OA. Of the 54 DH lines produced from the in vitro mutation-selection, two DH lines of resistant mutants, named M083 and M004, were selected following seedling and glasshouse tests. The resistance of M083 and M004 to S. sclerotiorum following tests with both mycelial inoculum and OA was greater than that of their donor lines and the resistant control Zhongyou 821. In both glasshouse and field disease nurseries, disease indices on M083 and M004 were less than 50% of those of the control. The time required for M083 and M004 to mature was 14 days and 10 days shorter, respectively, than that of their donor lines. Furthermore, M083 had more pods per inflorescence, a greater 1,000 seed weight and higher yield than its donor line. Random amplified polymorphic DNA characterisation showed that M083 had DNA band patterns that differed from its donor line.en
dc.relation.ispartofPlant Cell Reports
dc.subjectBrassica napus
dc.subjecthaploid somatic mutation and selection
dc.subjectSclerotinia sclerotiorum
dc.titleIn vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorumen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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