dc.contributor.author | Liao, Y. H. | |
dc.contributor.author | Jones, Stuart A. | |
dc.contributor.author | Nazir, T. | |
dc.contributor.author | Martin, Gary P. | |
dc.contributor.author | Brown, Marc | |
dc.date.accessioned | 2013-04-11T13:29:36Z | |
dc.date.available | 2013-04-11T13:29:36Z | |
dc.date.issued | 2005-11-04 | |
dc.identifier.citation | Liao , Y H , Jones , S A , Nazir , T , Martin , G P & Brown , M 2005 , ' The effects of polyvinyl alcohol on the in vitro stability and delivery of spray-dried protein particles from surfactant-free HFA 134a-based pressurised metered dose inhalers ' , International Journal of Pharmaceutics , vol. 304 , no. 1-2 , pp. 29-39 . https://doi.org/10.1016/j.ijpharm.2005.07.013 | |
dc.identifier.issn | 0378-5173 | |
dc.identifier.uri | http://hdl.handle.net/2299/10363 | |
dc.description.abstract | The objective of the present study was to investigate the physical stability of spray-dried proteins within surfactant-free hydrofluoroalkane (HFA) pressurised metered dose inhalers (pMDIs) during prolonged storage. Two model proteins (lysozyme and catalase) were spray-dried and stabilised in the presence of excipients, and subsequently suspended within HFA 134a. The pMDIs were stored valve-up for 6 months at room temperature (ca. 25 degrees C). Activities of the proteins were determined using biological assays and the fine particle fraction of the pMDIs was measured using a twin-stage impinger. The biological activities of catalase and lysozyme were found to be preserved in the presence of sugars and/or 80% hydrolysed polyvinyl alcohol (PVA) during spray drying. In addition, suspending the stabilised proteins within HFA for up to 6 months had little effect on their activity. The aerosolisation performance of lysozyme or catalase formulations containing either sucrose or trehalose as stabilisers appeared to deteriorate as a function of storage time. However, those formulations containing PVA were found to generate the greatest fine particle fraction, which in some cases was up to 50%, and to possess excellent physical stability during storage. The results indicated that the presence of PVA in the spray-dried stabilised protein particles could enhance the physical stability of particles, when suspended in the surfactant-free HFA MDI formulations, without affecting the protein stability upon prolonged storage. (c) 2005 Elsevier B.V All rights reserved. | en |
dc.format.extent | 11 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Pharmaceutics | |
dc.subject | spray-drying | |
dc.subject | protein stability | |
dc.subject | metered dose inhaler | |
dc.subject | pulmonary delivery | |
dc.subject | catalase | |
dc.subject | polyvinyl alcohol | |
dc.subject | lysozyme | |
dc.subject | hydrofluoroalkane | |
dc.subject | THERAPEUTIC PROTEINS | |
dc.subject | PRESERVATION | |
dc.subject | FORMULATION | |
dc.subject | SUSPENSION | |
dc.subject | LYSOZYME | |
dc.subject | LUNG | |
dc.title | The effects of polyvinyl alcohol on the in vitro stability and delivery of spray-dried protein particles from surfactant-free HFA 134a-based pressurised metered dose inhalers | en |
dc.contributor.institution | Department of Pharmacy | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Centre for Research into Topical Drug Delivery and Toxicology | |
dc.contributor.institution | Pharmaceutics | |
dc.contributor.institution | Skin and Nail Group | |
dc.contributor.institution | Airway Group | |
dc.contributor.institution | Bioadhesive Drug Delivery Group | |
dc.contributor.institution | Nanopharmaceutics | |
dc.contributor.institution | Pharmaceutical Analysis and Product Characterisation | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1016/j.ijpharm.2005.07.013 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |