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dc.contributor.authorLiao, Y. H.
dc.contributor.authorJones, Stuart A.
dc.contributor.authorNazir, T.
dc.contributor.authorMartin, Gary P.
dc.contributor.authorBrown, Marc
dc.date.accessioned2013-04-11T13:29:36Z
dc.date.available2013-04-11T13:29:36Z
dc.date.issued2005-11-04
dc.identifier.citationLiao , Y H , Jones , S A , Nazir , T , Martin , G P & Brown , M 2005 , ' The effects of polyvinyl alcohol on the in vitro stability and delivery of spray-dried protein particles from surfactant-free HFA 134a-based pressurised metered dose inhalers ' International Journal of Pharmaceutics , vol. 304 , no. 1-2 , pp. 29-39 . https://doi.org/10.1016/j.ijpharm.2005.07.013
dc.identifier.issn0378-5173
dc.identifier.otherPURE: 628125
dc.identifier.otherPURE UUID: 108a4986-a98c-441c-9860-76824731b654
dc.identifier.otherWOS: 000233126200005
dc.identifier.otherScopus: 26444484541
dc.identifier.urihttp://hdl.handle.net/2299/10363
dc.description.abstractThe objective of the present study was to investigate the physical stability of spray-dried proteins within surfactant-free hydrofluoroalkane (HFA) pressurised metered dose inhalers (pMDIs) during prolonged storage. Two model proteins (lysozyme and catalase) were spray-dried and stabilised in the presence of excipients, and subsequently suspended within HFA 134a. The pMDIs were stored valve-up for 6 months at room temperature (ca. 25 degrees C). Activities of the proteins were determined using biological assays and the fine particle fraction of the pMDIs was measured using a twin-stage impinger. The biological activities of catalase and lysozyme were found to be preserved in the presence of sugars and/or 80% hydrolysed polyvinyl alcohol (PVA) during spray drying. In addition, suspending the stabilised proteins within HFA for up to 6 months had little effect on their activity. The aerosolisation performance of lysozyme or catalase formulations containing either sucrose or trehalose as stabilisers appeared to deteriorate as a function of storage time. However, those formulations containing PVA were found to generate the greatest fine particle fraction, which in some cases was up to 50%, and to possess excellent physical stability during storage. The results indicated that the presence of PVA in the spray-dried stabilised protein particles could enhance the physical stability of particles, when suspended in the surfactant-free HFA MDI formulations, without affecting the protein stability upon prolonged storage. (c) 2005 Elsevier B.V All rights reserved.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofInternational Journal of Pharmaceutics
dc.subjectspray-drying
dc.subjectprotein stability
dc.subjectmetered dose inhaler
dc.subjectpulmonary delivery
dc.subjectcatalase
dc.subjectpolyvinyl alcohol
dc.subjectlysozyme
dc.subjecthydrofluoroalkane
dc.subjectTHERAPEUTIC PROTEINS
dc.subjectPRESERVATION
dc.subjectFORMULATION
dc.subjectSUSPENSION
dc.subjectLYSOZYME
dc.subjectLUNG
dc.titleThe effects of polyvinyl alcohol on the in vitro stability and delivery of spray-dried protein particles from surfactant-free HFA 134a-based pressurised metered dose inhalersen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionPharmaceutics
dc.contributor.institutionSkin and Nail Group
dc.contributor.institutionAirway Group
dc.contributor.institutionBioadhesive Drug Delivery Group
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.ijpharm.2005.07.013
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstyperestrictedAccess


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