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dc.contributor.authorEckert, M. R.
dc.contributor.authorGout, L.
dc.contributor.authorRouxel, Thierry
dc.contributor.authorBlaise, F
dc.contributor.authorJedryczka, M.
dc.contributor.authorFitt, Bruce D.L.
dc.contributor.authorBalesdent, Marie-Helene
dc.identifier.citationEckert , M R , Gout , L , Rouxel , T , Blaise , F , Jedryczka , M , Fitt , B D L & Balesdent , M-H 2005 , ' Identification and characterization of polymorphic minisatellites in the phytopathogenic ascomycete Leptosphaeria maculans ' , Current Genetics , vol. 47 , no. 1 , pp. 37-48 .
dc.identifier.otherPURE: 1388851
dc.identifier.otherPURE UUID: 60eb46bd-a5ae-4dea-8d33-62209975bdd5
dc.identifier.otherScopus: 11244285207
dc.description.abstractLeptosphaeria maculans causes phoma stem canker, the most serious disease of oilseed rape world-wide. Sexual recombination is important in the pathogen life cycle and increases the risk of plant resistance genes being overcome rapidly. Thus, there is a need to develop easy-to-use molecular markers suitable for large-scale population genetic studies. The minisatellite MinLm1, showing six alleles in natural populations, has previously been used as a marker to survey populations. Here, we report the characterization of five new minisatellites (MinLm2-MinLm6), of which four were identified by a systematic search for tandemly repeated polymorphic regions in BAC-end sequencing data from L. maculans. Of 782 BAC-end sequences analysed, 43 possessed putative minisatellite-type repeats and four of these (MinLm3-MinLm6) displayed both consistent PCR amplification and size polymorphism in a collection of L. maculans isolates of diverse origins. Cloning and sequencing of each allele confirmed that polymorphism was due to variation in the repeat number of a core motif ranging from 11 bp (MinLm3) to 51 bp (MinLm4). The number of alleles found for each minisatellite ranged from three (MinLm4) to nine (MinLm2), with eight, five and six for MinLm3, MinLm5 and MinLm6, respectively. MinLm2-MinLm6 are all single locus markers specific to L. maculans and share some common features, such as conservation of core motifs and incomplete direct repeats in the flanking regions. To our knowledge, L. maculans is the first fungal species for which six polymorphic single locus minisatellite markers have been reporteden
dc.relation.ispartofCurrent Genetics
dc.subjectPhoma lingam
dc.subjectvariable number tandem repeat locus
dc.subjectDNA fingerprinting
dc.subjecttandem repeat
dc.subjectpopulation genetics
dc.titleIdentification and characterization of polymorphic minisatellites in the phytopathogenic ascomycete Leptosphaeria maculansen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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