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dc.contributor.authorMkrtchyan, Hermine
dc.contributor.authorGibbons, Simon
dc.contributor.authorHeidelberger, Sibylle
dc.contributor.authorZloh, Mire
dc.contributor.authorLimaki, Hamidreza Khalatbari
dc.identifier.citationMkrtchyan , H , Gibbons , S , Heidelberger , S , Zloh , M & Limaki , H K 2010 , ' Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narine ' , International Journal of Antimicrobial Agents , vol. 35 , no. 3 , pp. 255-60 .
dc.identifier.otherPURE: 1457556
dc.identifier.otherPURE UUID: 8ac5ac6d-38b4-4239-b749-1243176be360
dc.identifier.otherPubMed: 20045288
dc.identifier.otherScopus: 73549120400
dc.descriptionCopyright 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
dc.description.abstractIn the last two decades, antimicrobial peptides (AMPs) have been gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Lactobacillus acidophilus n.v. Er 317/402 strain Narine produces a small AMP with a molecular weight of 1.1kDa, designated acidocin LCHV. In this study, the AMP was extremely heat stable (90min at 130 degrees C), was active over a wide pH range and was found to be sensitive to proteolytic enzymes (trypsin, pepsin and proteinase K). Acidocin LCHV has a broad spectrum of activity both against Gram-positive and Gram-negative pathogens, including several that are classified as Especially Dangerous Infections by the World Health Organization as well as meticillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) was used to determine the molecular mass and sequence of the purified peptide. Complete killing with immediate impact on cells was observed within a very short period of time (10min).en
dc.relation.ispartofInternational Journal of Antimicrobial Agents
dc.titlePurification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narineen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionMedicinal and Analytical Chemistry
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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