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dc.contributor.authorWard, E.
dc.contributor.authorGray, R. M.
dc.date.accessioned2013-04-30T09:34:59Z
dc.date.available2013-04-30T09:34:59Z
dc.date.issued1992-12
dc.identifier.citationWard , E & Gray , R M 1992 , ' Generation of a ribosomal DNA probe by PCR and its use in identification of fungi within the Gaeumannomyces-Phialophora complex ' , Plant Pathology , vol. 41 , no. 6 , pp. 730-736 . https://doi.org/10.1111/j.1365-3059.1992.tb02556.x
dc.identifier.issn0032-0862
dc.identifier.otherPURE: 1271732
dc.identifier.otherPURE UUID: 390e5950-b479-4ba9-9da1-e109a3d51fdd
dc.identifier.otherWOS: A1992KD34100008
dc.identifier.otherScopus: 84987048006
dc.identifier.urihttp://hdl.handle.net/2299/10613
dc.description.abstractThe polymerase chain reaction (PCR) was used to amplify a ribosomal DNA fragment from Gaewnannomyces graminis. This fragment was labelled and tested for its usefulness as a probe in restriction fragment length polymorphism (RFLP) studies of fungi within the Gaeumannomyces-Phialophora complex. When the probe was hybridized to EcoRI digests of DNA from these fungi, there were consistent band pattern differences between the three varieties of G. graminis (tritici, avenae and graminis). This method of probe production, which is more rapid than many others currently used, has considerable potential for use in the identification of these organisms, and may also be applicable to other groups of fungi.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofPlant Pathology
dc.titleGeneration of a ribosomal DNA probe by PCR and its use in identification of fungi within the Gaeumannomyces-Phialophora complexen
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1111/j.1365-3059.1992.tb02556.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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