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        A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum

        Author
        Freeman, J.
        Ward, Elaine
        Calderon, C.
        McCartney, A.
        Attention
        2299/10630
        Abstract
        The development of a polymerase chain reaction (PCR) assay for the detection of inoculum of the plant pathogenic fungus Sclerotinia sclerotiorum is described. The PCR primers were designed using nuclear ribosomal DNA internal transcribed spacer sequences. Specific detection of DNA from S. sclerotiorum was possible even in the presence of a 40-fold excess of DNA from the closely related fungus Botrytis cinerea. PCR products were obtained from suspensions of untreated S. sclerotiorum ascospores alone, but DNA purification was required for detection in the presence of large numbers of B. cinerea conidiospores. Specific detection of inoculum of S. sclerotiorum was possible in field-based air-samples, using a Burkard spore trap, and from inoculated oilseed rape petals. The assay has potential for incorporation into a risk management system for S. sclerotiorum in oilseed rape crops
        Publication date
        2002-11
        Published in
        European Journal of Plant Pathology
        Published version
        https://doi.org/10.1023/A:1021216720024
        Other links
        http://hdl.handle.net/2299/10630
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