dc.contributor.author | Rashid, T. | |
dc.contributor.author | Banerjee, M. | |
dc.contributor.author | Nikolic, M. | |
dc.date.accessioned | 2013-05-09T07:15:01Z | |
dc.date.available | 2013-05-09T07:15:01Z | |
dc.date.issued | 2001-12-28 | |
dc.identifier.citation | Rashid , T , Banerjee , M & Nikolic , M 2001 , ' Phosphorylation of Pak1 by the p35/Cdk5 Kinase Affects Neuronal Morphology ' , Journal of Biological Chemistry , vol. 276 , no. 52 , pp. 49043-49052 . https://doi.org/10.1074/jbc.M105599200 | |
dc.identifier.issn | 0021-9258 | |
dc.identifier.uri | http://hdl.handle.net/2299/10656 | |
dc.description.abstract | The small GTPase Rac and its effectors, the Pak1 and p35/Cdk5 kinases, have been assigned important roles in regulating cytoskeletal dynamics in neurons. Our previous work revealed that the neuronal p35/Cdk5 kinase associates with Pak1 in a RacGTP-dependent manner, causing hyperphosphorylation and down-regulation of Pak1 kinase activity. We have now demonstrated direct phosphorylation of Pak1 on threonine 212 by the p35/ Cdk5 kinase. In neuronal growth cones, Pak1 phosphorylated on Thr-212 localized to actin and tubulin-rich areas, suggesting a role in regulating growth cone dynamics. The expression of a non-phosphorylatable Pak1 mutant (Pak1A212) induced dramatic neurite disorganization. We also observed a strong association between p35/Cdk5 and the Pak1 C-terminal kinase domain. Overall, our data show that in neurons, membrane-associated, active Pak1 is regulated by the p35/Cdk5 kinase both by association and phosphorylation, which is essential for the proper regulation of the cytoskeleton during neurite outgrowth and remodeling. | en |
dc.format.extent | 10 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Biological Chemistry | |
dc.title | Phosphorylation of Pak1 by the p35/Cdk5 Kinase Affects Neuronal Morphology | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=0035966018&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1074/jbc.M105599200 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |