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dc.contributor.authorFould, S.
dc.contributor.authorDieng, A. L.
dc.contributor.authorDavies, Keith
dc.contributor.authorNormand, P.
dc.contributor.authorMateille, T.
dc.date.accessioned2013-05-09T08:05:01Z
dc.date.available2013-05-09T08:05:01Z
dc.date.issued2001-11
dc.identifier.citationFould , S , Dieng , A L , Davies , K , Normand , P & Mateille , T 2001 , ' Immunological quantification of the nematode parasitic bacterium Pasteuria penetrans in soil ' , FEMS Microbiology Ecology , vol. 37 , no. 3 , pp. 187-195 . https://doi.org/10.1111/j.1574-6941.2001.tb00866.x
dc.identifier.issn0168-6496
dc.identifier.otherPURE: 590339
dc.identifier.otherPURE UUID: c0274226-6e1f-49c5-9518-24cbfe44ae73
dc.identifier.otherWOS: 000172743300001
dc.identifier.otherScopus: 0035216154
dc.identifier.urihttp://hdl.handle.net/2299/10661
dc.description.abstractCurrently, the abundance of Pasteuria penetrans in soils, an unculturable bacterial parasite of root-knot nematodes (Meloidogyne spp,). is estimated by the percentage of nematode juveniles infected with bacteria and the number of spores attached to their cuticle. Indirect immunofluorescence led to detection of free spores directly in soil suspensions using UV light and polyclonal antibodies raised against two P. penetrans populations (ORS-21414-Sen and PPI). Three extraction methods were compared in order to improve spore recovery. A gentle shaking/sieving method recovered more than 90% of the spores inoculated in soils and was more efficient and simple than aqueous two-phase partitioning and polyethylene glycol extractions. All the spores inoculated in sandy or sandy-clay soils were detected with immunofluorescence microscopy. The quantification of the spores was improved using an ELISA technique that showed a good correlation between optical density and spore concentration in inoculated soils. Specific antibodies provide a suitable method to quantify A penetrans and may be used to follow the evolution of the real pool of bacteria either in native suppressive soils or in inoculated ones. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofFEMS Microbiology Ecology
dc.subjectimmunological detection
dc.subjectsoil
dc.subjectunculturable bacterium
dc.subjectPasteuria penetrans
dc.subjectROOT-KNOT NEMATODES
dc.subjectMELOIDOGYNE-INCOGNITA
dc.subjectHYPERPARASITIC BACTERIUM
dc.subjectPHYLOGENETIC POSITION
dc.subjectWHEAT RHIZOSPHERE
dc.subjectCYST-NEMATODE
dc.subjectLIFE-CYCLE
dc.subjectSP-NOV
dc.subjectSPORES
dc.subjectHETERODERA
dc.titleImmunological quantification of the nematode parasitic bacterium Pasteuria penetrans in soilen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2001-11
rioxxterms.versionofrecordhttps://doi.org/10.1111/j.1574-6941.2001.tb00866.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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