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dc.contributor.authorMohan, S.
dc.contributor.authorFould, S.
dc.contributor.authorDavies, Keith
dc.date.accessioned2013-05-09T08:10:00Z
dc.date.available2013-05-09T08:10:00Z
dc.date.issued2001-09
dc.identifier.citationMohan , S , Fould , S & Davies , K 2001 , ' The interaction between the gelatin-binding domain of fibronectin and the attachment of Pasteuria penetrans endospores to nematode cuticle ' , Parasitology , vol. 123 , no. 3 , pp. 271-276 . https://doi.org/10.1017/S0031182001008411
dc.identifier.issn0031-1820
dc.identifier.otherPURE: 590385
dc.identifier.otherPURE UUID: c44f6063-7dd7-4969-8d3f-d638a4887cee
dc.identifier.otherWOS: 000171485400007
dc.identifier.otherScopus: 0034835371
dc.identifier.urihttp://hdl.handle.net/2299/10663
dc.description.abstractPasteuria Penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kDa heparin-binding domain (HBD) and the 45 kDa gelatin-binding domain (GBD) fragments of human fibronectin (Fn) revealed a series of polypeptides of approximately 40, 45 and 55 kDa present in crude cuticle extracts of Meloidogyne javanica 2nd-stage juveniles. The results suggest that the structure of the nematode fibronectin is different to the fibronectins so far characterized. Pre-treatment of endospores of Pasteuria with either the HBD or the GBD was found to inhibit binding to the nematode cuticle. The larger GBD fragment was the most effective at blocking adhesion. Pre-treatment of the GBD fragment with gelatin prevented the GBD fragment from inhibiting endospore attachment to the nematode cuticle.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofParasitology
dc.subjectnematoda
dc.subjectMeloidogyne javanica
dc.subjectbacterial adhesion
dc.subjectextracellular matrix
dc.subjectbiological control
dc.subjectMELOIDOGYNE-INCOGNITA
dc.subjectJUVENILES
dc.subjectVIRULENCE
dc.subjectADHESION
dc.subjectFEMALES
dc.subjectSURFACE
dc.titleThe interaction between the gelatin-binding domain of fibronectin and the attachment of Pasteuria penetrans endospores to nematode cuticleen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2001-09
rioxxterms.versionofrecordhttps://doi.org/10.1017/S0031182001008411
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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