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dc.contributor.authorChilcott, Robert
dc.contributor.authorDalton, C. H.
dc.contributor.authorHill, I.
dc.contributor.authorDavison, C. M.
dc.contributor.authorBlohm, K. L.
dc.contributor.authorClarkson, E. D.
dc.contributor.authorHamilton, M. G.
dc.date.accessioned2013-06-05T12:30:51Z
dc.date.available2013-06-05T12:30:51Z
dc.date.issued2005-07
dc.identifier.citationChilcott , R , Dalton , C H , Hill , I , Davison , C M , Blohm , K L , Clarkson , E D & Hamilton , M G 2005 , ' In vivo skin absorption and distribution of the nerve agent VX (O-ethyl-S-[2(diisopropylamino)ethyl] methylphosphonothioate) in the domestic white pig ' , Human & Experimental Toxicology , vol. 24 , no. 7 , pp. 347-352 . https://doi.org/10.1191/0960327105ht537oa
dc.identifier.issn0960-3271
dc.identifier.otherPURE: 678278
dc.identifier.otherPURE UUID: b5748e54-ac70-4523-97ff-cb18019936e4
dc.identifier.otherWOS: 000231247900002
dc.identifier.otherScopus: 23744504223
dc.identifier.urihttp://hdl.handle.net/2299/10721
dc.description.abstractThe purpose of this study was to characterize the skin absorption and distribution of VX (O-ethyl-S-[2 (diisopropylamino)ethyl] methylphosphonothioate) in the domestic pig in order to evaluate the animal as a potential model for assessing pretreatments against toxic anti-cholinesterase compounds. A liquid droplet (equivalent to a 2 x LD50 dose) of radiolabelled VX was applied to the inner ear-skin of each anaesthetized animal. Blood and tissue samples (liver, lung, kidney, heart and skin exposure sites) were obtained post-mortem. The amount of radioactivity in each sample was measured by liquid scintillation counting, from which the skin absorption rate and dose distribution of VX were calculated. A substantial proportion (22 +/- 3%) of the applied dose remained within the skin at the site of application. It is conceivable that strategies to minimize or remove this reservoir may be of benefit in the early treatment of VX-exposed casualties. Image analysis of autoradiographs of exposed skin sites indicated that each milligram of radioactive VX covered an area of 1.2 +/- 0.5 cm(2). The average skin absorption rate of C-14-VX was 661 +/- 12 6 mu g/cm(2) per hour. Comparison of these data with previous studies suggests that human skin is less permeable to VX than pig skin, but VX spreads over a greater surface area when applied to human skin. Thus, paradoxically, while pig-ear skin is more permeable than human skin, the difference in skin surface spreading may lead to the absorption of an equivalent systemic dose.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofHuman & Experimental Toxicology
dc.subjectanti-cholinesterase
dc.subjectin vivo
dc.subjectpig
dc.subjectPERCUTANEOUS-ABSORPTION
dc.subjectSULFUR MUSTARD
dc.subjectEAR SKIN
dc.subjectVITRO
dc.subjectPERMEABILITY
dc.subjectBLOOD
dc.titleIn vivo skin absorption and distribution of the nerve agent VX (O-ethyl-S-[2(diisopropylamino)ethyl] methylphosphonothioate) in the domestic white pigen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionPharmaceutics
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionToxicology
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2005-07
rioxxterms.versionofrecordhttps://doi.org/10.1191/0960327105ht537oa
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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