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dc.contributor.authorKaratolos, Nikos
dc.contributor.authorWilliamson, M. S.
dc.contributor.authorDenholm, Ian
dc.contributor.authorGorman, Kevin
dc.contributor.authorFfrench-Constant, R.
dc.contributor.authorNauen, Ralf
dc.date.accessioned2013-06-17T13:00:44Z
dc.date.available2013-06-17T13:00:44Z
dc.date.issued2012-06
dc.identifier.citationKaratolos , N , Williamson , M S , Denholm , I , Gorman , K , Ffrench-Constant , R & Nauen , R 2012 , ' Resistance to spiromesifen in Trialeurodes vaporariorum is associated with a single amino acid replacement in its target enzyme acetyl-coenzyme A carboxylase ' , Insect Biochemistry and Molecular Biology , vol. 21 , no. 3 , pp. 327-334 . https://doi.org/10.1111/j.1365-2583.2012.01136.x
dc.identifier.issn0965-1748
dc.identifier.otherPURE: 1935251
dc.identifier.otherPURE UUID: ae45fdc4-7eb8-451f-bafb-8f7010ccb5d8
dc.identifier.otherWOS: 000303796000005
dc.identifier.otherScopus: 84861094324
dc.identifier.otherORCID: /0000-0001-9797-874X/work/35873684
dc.identifier.urihttp://hdl.handle.net/2299/10790
dc.description.abstractSpiromesifen is a novel insecticide and is classed as a tetronic acid derivative. It targets the insects' acetyl-coenzyme A carboxylase (ACCase) enzyme, causing a reduction in lipid biosynthesis. At the time of this publication, there are no reports of resistance to this class of insecticides in insects although resistance has been observed in several mite species. The greenhouse whitefly Trialeurodes vaporariorum (Westwood) is a serious pest of protected vegetable and ornamental crops in temperate regions of the world and spiromesifen is widely used in its control. Mortality rates of UK and European populations of T. vaporariorum to spiromesifen were calculated and up to 26-fold resistance was found. We therefore sought to examine the molecular mechanism underlying spiromesifen resistance in this important pest. Pre-treatment with piperonyl butoxide did not synergize spiromesifen, suggesting a target-site resistance mechanism. The full length ACCase gene was sequenced for a range of T. vaporariorum strains and a strong association was found between spiromesifen resistance and a glutamic acid substitution with lysine in position 645 (E645K) of this gene. A TaqMan allelic discrimination assay confirmed these findings. Although this resistance is not considered sufficient to compromise the field performance of spiromesifen, this association of E645K with resistance is the first report of a potential target site mechanism affecting an ACCase inhibitor in an arthropod species.en
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofInsect Biochemistry and Molecular Biology
dc.subjectSUSCEPTIBILITY
dc.subjectSTRAIN
dc.subjectMITES
dc.subjectwhiteflies
dc.subjectSPIRODICLOFEN
dc.subjectALEYRODIDAE
dc.subjectCOA-CARBOXYLASE
dc.subjectHEMIPTERA
dc.subjectacetyl-coenzyme A carboxylase
dc.subjectINSECTICIDE
dc.subjectDOMAIN
dc.subjectKOCH ACARI TETRANYCHIDAE
dc.subjectspiromesifen resistance
dc.titleResistance to spiromesifen in Trialeurodes vaporariorum is associated with a single amino acid replacement in its target enzyme acetyl-coenzyme A carboxylaseen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionEcology
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1111/j.1365-2583.2012.01136.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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