University of Hertfordshire Research Archive

        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of UHRABy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

        Arkivum Files

        My Downloads
        View Item 
        • UHRA Home
        • University of Hertfordshire
        • Research publications
        • View Item
        • UHRA Home
        • University of Hertfordshire
        • Research publications
        • View Item

        Identification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterases

        Author
        Ibrahim, S. K.
        Davies, Keith
        Perry, R. N.
        Attention
        2299/10800
        Abstract
        The esterase isozyme from Meloidogyne incognita with pI similar to 4.95 was purified using a Rotofor electrophoresis system. The purified enzyme was then used to raise monoclonal antibodies. Hybridoma cell lines producing antibodies were cloned and screened against the purified esterase in an enzyme-linked immunosorbent assay (ELISA). A selected monoclonal antibody was then tested by western blot analysis of crude soluble protein extracts of all the major species of Meloidogyne separated by native and SDS-PAGE electrophoresis. Although the antibody was cross-reactive in western blots of denatured proteins in the presence of SDS and in ELISA, it was not cross-reactive in western blots of native protein. The results demonstrate that a monoclonal antibody to a diagnostic non-specific esterase enables M. incognita to be discriminated from M. javanica without the need to separate the esterases by electrophoresis first. This is an approach which could be adopted in a number of situations where organisms can be identified by a diagnostic protein which has already been identified by electrophoresis
        Publication date
        1996-08
        Published in
        Physiological and Molecular Plant Pathology
        Published version
        https://doi.org/10.1006/pmpp.1996.0041
        Other links
        http://hdl.handle.net/2299/10800
        Metadata
        Show full item record
        Keep in touch

        © 2019 University of Hertfordshire

        I want to...

        • Apply for a course
        • Download a Prospectus
        • Find a job at the University
        • Make a complaint
        • Contact the Press Office

        Go to...

        • Accommodation booking
        • Your student record
        • Bayfordbury
        • KASPAR
        • UH Arts

        The small print

        • Terms of use
        • Privacy and cookies
        • Criminal Finances Act 2017
        • Modern Slavery Act 2015
        • Sitemap

        Find/Contact us

        • T: +44 (0)1707 284000
        • E: ask@herts.ac.uk
        • Where to find us
        • Parking
        • hr
        • qaa
        • stonewall
        • AMBA
        • ECU Race Charter
        • disability confident
        • AthenaSwan