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dc.contributor.authorIbrahim, S. K.
dc.contributor.authorDavies, Keith
dc.contributor.authorPerry, R. N.
dc.date.accessioned2013-06-17T14:30:42Z
dc.date.available2013-06-17T14:30:42Z
dc.date.issued1996-08
dc.identifier.citationIbrahim , S K , Davies , K & Perry , R N 1996 , ' Identification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterases ' , Physiological and Molecular Plant Pathology , vol. 49 , no. 2 , pp. 79-88 . https://doi.org/10.1006/pmpp.1996.0041
dc.identifier.issn0885-5765
dc.identifier.otherPURE: 590925
dc.identifier.otherPURE UUID: 81895d3f-1382-4dde-b261-0a97f2775b24
dc.identifier.otherWOS: A1996VM77700002
dc.identifier.otherScopus: 0030207231
dc.identifier.otherORCID: /0000-0001-6060-2394/work/32215822
dc.identifier.urihttp://hdl.handle.net/2299/10800
dc.description.abstractThe esterase isozyme from Meloidogyne incognita with pI similar to 4.95 was purified using a Rotofor electrophoresis system. The purified enzyme was then used to raise monoclonal antibodies. Hybridoma cell lines producing antibodies were cloned and screened against the purified esterase in an enzyme-linked immunosorbent assay (ELISA). A selected monoclonal antibody was then tested by western blot analysis of crude soluble protein extracts of all the major species of Meloidogyne separated by native and SDS-PAGE electrophoresis. Although the antibody was cross-reactive in western blots of denatured proteins in the presence of SDS and in ELISA, it was not cross-reactive in western blots of native protein. The results demonstrate that a monoclonal antibody to a diagnostic non-specific esterase enables M. incognita to be discriminated from M. javanica without the need to separate the esterases by electrophoresis first. This is an approach which could be adopted in a number of situations where organisms can be identified by a diagnostic protein which has already been identified by electrophoresisen
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofPhysiological and Molecular Plant Pathology
dc.subjectCYST NEMATODES
dc.subjectPHENOTYPES
dc.subjectPROTEIN
dc.subjectFEMALES
dc.subjectELECTROPHORESIS
dc.subjectQUANTITIES
dc.subjectPATTERNS
dc.subjectRICE
dc.titleIdentification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterasesen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1006/pmpp.1996.0041
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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