dc.contributor.author | Ibrahim, S. K. | |
dc.contributor.author | Davies, Keith | |
dc.contributor.author | Perry, R. N. | |
dc.date.accessioned | 2013-06-17T14:30:42Z | |
dc.date.available | 2013-06-17T14:30:42Z | |
dc.date.issued | 1996-08 | |
dc.identifier.citation | Ibrahim , S K , Davies , K & Perry , R N 1996 , ' Identification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterases ' , Physiological and Molecular Plant Pathology , vol. 49 , no. 2 , pp. 79-88 . https://doi.org/10.1006/pmpp.1996.0041 | |
dc.identifier.issn | 0885-5765 | |
dc.identifier.other | ORCID: /0000-0001-6060-2394/work/32215822 | |
dc.identifier.uri | http://hdl.handle.net/2299/10800 | |
dc.description.abstract | The esterase isozyme from Meloidogyne incognita with pI similar to 4.95 was purified using a Rotofor electrophoresis system. The purified enzyme was then used to raise monoclonal antibodies. Hybridoma cell lines producing antibodies were cloned and screened against the purified esterase in an enzyme-linked immunosorbent assay (ELISA). A selected monoclonal antibody was then tested by western blot analysis of crude soluble protein extracts of all the major species of Meloidogyne separated by native and SDS-PAGE electrophoresis. Although the antibody was cross-reactive in western blots of denatured proteins in the presence of SDS and in ELISA, it was not cross-reactive in western blots of native protein. The results demonstrate that a monoclonal antibody to a diagnostic non-specific esterase enables M. incognita to be discriminated from M. javanica without the need to separate the esterases by electrophoresis first. This is an approach which could be adopted in a number of situations where organisms can be identified by a diagnostic protein which has already been identified by electrophoresis | en |
dc.format.extent | 10 | |
dc.language.iso | eng | |
dc.relation.ispartof | Physiological and Molecular Plant Pathology | |
dc.subject | CYST NEMATODES | |
dc.subject | PHENOTYPES | |
dc.subject | PROTEIN | |
dc.subject | FEMALES | |
dc.subject | ELECTROPHORESIS | |
dc.subject | QUANTITIES | |
dc.subject | PATTERNS | |
dc.subject | RICE | |
dc.title | Identification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterases | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1006/pmpp.1996.0041 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |