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dc.contributor.authorGould, L. A.
dc.contributor.authorLansley, A. B.
dc.contributor.authorBrown, Marc
dc.contributor.authorForbes, B.
dc.contributor.authorMartin, Gary P.
dc.date.accessioned2013-06-19T08:30:44Z
dc.date.available2013-06-19T08:30:44Z
dc.date.issued2000-10
dc.identifier.citationGould , L A , Lansley , A B , Brown , M , Forbes , B & Martin , G P 2000 , ' Mitigation of surfactant erythrocyte toxicity by egg phosphatidylcholine ' , Journal of Pharmacy and Pharmacology , vol. 52 , no. 10 , pp. 1203-1209 .
dc.identifier.issn0022-3573
dc.identifier.otherPURE: 629409
dc.identifier.otherPURE UUID: 5357080f-9a0d-441b-a9b5-8df47853d7ed
dc.identifier.otherWOS: 000090079200005
dc.identifier.otherScopus: 0033765627
dc.identifier.urihttp://hdl.handle.net/2299/10851
dc.description.abstractPolyoxyethylene alkyl ether surfactants have been shown to have excellent penetration enhancing abilities although they are associated with a high level of local toxicity. We have compared the toxicity of a range of polyoxyethylene alkyl ethers (Brij 96, Brij 76, Brij 56, 10 lauryl ether and 9 lauryl ether) to an anionic surfactant (sodium dodecyl sulphate (SDS)), an ampholytic surfactant (lysophosphatidylcholine) and a cationic surfactant (tetradecyltrimethylammonium bromide (TTAB)), in the presence and absence of egg phosphatidylcholine. The toxicity of the surfactants or phospholipid/surfactant mixtures was assessed by measuring haemolytic activity. The test samples were incubated with a suspension of red blood cells for 30min and Drabkin's reagent was used to indicate the amount of haemoglobin released. All of the polyoxyethylene alkyl ethers, SDS, TTAB and lysophosphatidylcholine exhibited haemolytic activity at concentrations between 0.10 and 0.25 mM. The addition of egg phosphatidylcholine reduced the toxicity for all of the surfactants, with the toxicity of Brij 96 being mitigated to a greater extent than the toxicity of the other polyoxyethylene surfactants examined. The rate of haemolysis induced by Brij 96 or 10 lauryl ether was also reduced by increasing concentrations of phosphatidylcholine. As the phosphatidylcholine content of a mixed surfactant system comprising egg phosphatidylcholine : Brij 96 was replaced by lysophosphatidylcholine and fatty acid, the haemolytic action of the mixture increased markedly. The results from this study show that the toxicity of surfactants to erythrocytes can be mitigated by the addition of egg phosphatidylcholine. Synthetic surfactants combined with phosphatidylcholine may generate drug delivery systems worthy of more extensive investigation.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofJournal of Pharmacy and Pharmacology
dc.subjectNON-IONIC SURFACTANTS
dc.subjectMEMBRANE DAMAGE
dc.subjectBILE-SALTS
dc.subjectNASAL ABSORPTION
dc.subjectMIXED MICELLES
dc.subjectLYSOPHOSPHATIDYLCHOLINE
dc.subjectSOLUBILIZATION
dc.subjectENHANCEMENT
dc.titleMitigation of surfactant erythrocyte toxicity by egg phosphatidylcholineen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionPharmaceutics
dc.contributor.institutionSkin and Nail Group
dc.contributor.institutionAirway Group
dc.contributor.institutionBioadhesive Drug Delivery Group
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2000-10
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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