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dc.contributor.authorCovelli, L.
dc.contributor.authorHernández, C.
dc.contributor.authorFlores, R.
dc.contributor.authorRagozzino, A.
dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorDi Serio, F.
dc.contributor.authorCitir, A.
dc.contributor.authorAçikgöz, S.
dc.date.accessioned2013-06-24T08:15:58Z
dc.date.available2013-06-24T08:15:58Z
dc.date.issued2004-11-01
dc.identifier.citationCovelli , L , Hernández , C , Flores , R , Ragozzino , A , Coutts , R H A , Di Serio , F , Citir , A & Açikgöz , S 2004 , ' Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. I : Characterization of a new species in the genus Chrysovirus ' , Journal of General Virology , vol. 85 , no. 11 , pp. 3389-3397 . https://doi.org/10.1099/vir.0.80181-0
dc.identifier.issn0022-1317
dc.identifier.otherPURE: 1954584
dc.identifier.otherPURE UUID: 30ab2ea7-18e3-4b4a-8383-909040705cd1
dc.identifier.otherScopus: 7444238257
dc.identifier.urihttp://hdl.handle.net/2299/10904
dc.description.abstractCherry chlorotic rusty spot (CCRS) and Amasya cherry disease (ACD) display similar symptoms and are associated with a series of dsRNAs. However, a direct comparison has been lacking. Here, a side-by-side analysis confirmed that both diseases were symptomatologically very similar, as were the number (10-12) and size of their associated dsRNAs. Sequence determination of four of these dsRNAs revealed that they were essentially identical for CCRS and ACD. The largest (3399 bp), which potentially encoded a protein of 1087 aa with the eight motifs conserved in RNA-dependent RNA polymerases of dsRNA mycoviruses, had the highest similarity to those coded by dsRNA 1 of viruses belonging to the genus Chrysovirus and was termed CCRS or ACD chrys-dsRNA 1. The three closely migrating dsRNAs had the properties of the other components of a chrysovirus and in CCRS and ACD versions, respectively, were chrys-dsRNA 2 (3125 and 3128 bp), chrys-dsRNA 3 (2833 bp) and chrys-dsRNA 4 (2499 and 2498 bp), potentially encoding the major capsid protein (993 and 994 aa) and two proteins (884 and 677 aa, respectively) of unknown function. The four 5′- and 3′-UTRs shared internal similarities and had conserved GAAAAUUAUGG and AUAUGC termini, respectively. The 5′-UTRs contained the 'Box 1' motif followed by a stretch rich in CAA, CAAA and CAAAA repeats, characteristic of chrysovirus dsRNAs. Because species of the genus Chrysovirus have only been described as infecting fungi, this suggests a fungal aetiology for CCRS and ACD, a proposal supported by the properties of two other CCRS- and ACD-associated dsRNAs (see accompanying paper by Coutts et al., 2004, in this issue).en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofJournal of General Virology
dc.titleCherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. I : Characterization of a new species in the genus Chrysovirusen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=7444238257&partnerID=8YFLogxK
rioxxterms.versionofrecordhttps://doi.org/10.1099/vir.0.80181-0
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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