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dc.contributor.authorLivieratos, I.C.
dc.contributor.authorEliasco, E.
dc.contributor.authorMüller, G.
dc.contributor.authorSalazar, L.F.
dc.contributor.authorOlsthoorn, R.C.L.
dc.contributor.authorPleij, C.W.A.
dc.contributor.authorCoutts, Robert H.A.
dc.identifier.citationLivieratos , I C , Eliasco , E , Müller , G , Salazar , L F , Olsthoorn , R C L , Pleij , C W A & Coutts , R H A 2004 , ' Analysis of the RNA of Potato yellow vein virus : Evidence for a tripartite genome and conserved 3′-terminal structures among members of the genus Crinivirus ' , Journal of General Virology , vol. 85 , no. 7 , pp. 2065-2075 .
dc.identifier.otherPURE: 1957873
dc.identifier.otherPURE UUID: ad57afe7-8756-4b22-9a48-865f982194ac
dc.identifier.otherScopus: 3342912345
dc.description.abstractDouble-stranded RNA preparations produced from potato plants graft-inoculated with a Peruvian isolate of Potato yellow vein virus (PYVV; genus Crinivirus, family Closteroviridae) contain five RNA species denoted RNA 1, RNA 2, RNA 3, x and y of approximately 8, 5.3, 3.8, 2.0 and 1.8 kbp, respectively. The complete nucleotide sequences of PYVV RNAs 1, 2 and 3 and Northern hybridization analysis showed that PYVV RNA 1 contained the replication module and an additional open reading frame (p7), while two distinct species, RNAs 2 and 3, contain the Closteroviridae hallmark gene array. Pairwise comparisons and phylogeny of genome-encoded proteins showed that PYVV shares significant homology with other criniviruses but is most closely related to the Trialeurodes vaporariorum-vectored Cucumber yellows virus. Secondary structure prediction of the 3′-untranslated regions of all three PYVV RNAs revealed four conserved stem-loop structures and a 3′-terminal pseudoknot structure, also predicted for all fully characterized members of the genus Crinivirus and some members of the genera Closterovirus and Ampelovirus.en
dc.relation.ispartofJournal of General Virology
dc.titleAnalysis of the RNA of Potato yellow vein virus : Evidence for a tripartite genome and conserved 3′-terminal structures among members of the genus Crinivirusen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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