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dc.contributor.authorJones, Stuart A.
dc.contributor.authorMartin, Gary P.
dc.contributor.authorBrown, Marc
dc.date.accessioned2013-06-24T09:30:55Z
dc.date.available2013-06-24T09:30:55Z
dc.date.issued2004-05-28
dc.identifier.citationJones , S A , Martin , G P & Brown , M 2004 , ' Determination of polyvinylpyrrolidone using high-performance liquid chromatography ' , Journal of Pharmaceutical and Biomedical Analysis , vol. 35 , no. 3 , pp. 621-624 . https://doi.org/10.1016/j.jpba.2004.01.024
dc.identifier.issn0731-7085
dc.identifier.otherPURE: 628701
dc.identifier.otherPURE UUID: 4f56d20b-a16a-4743-95d5-c408ef9a3e20
dc.identifier.otherWOS: 000221710100022
dc.identifier.otherScopus: 2342650064
dc.identifier.urihttp://hdl.handle.net/2299/10913
dc.description.abstractPolyvinylpyrrolidone (PVP) is a versatile polymer with innate surface activity. It is very difficult to accurately assay due to its wide molecular weight range and amphiphilic nature. This study evaluated a reversed-phase HPLC method to separate and quantify PVP K15. The assay used a Hicrome C-18 150 mm x 3 mum HPLC column in combination with an 80/20 propanol-1-ol: deionised water, 0.01% TFA mobile phase, which resolved the polymer as a single peak, t(R) = 10.69 +/- 0.17 min (n = 120) at 243 nm. The column's performance was constant throughout the study, N (theoretical plates) = 1729 +/- 22 and the peak symmetry remained good (A(s) ranged from 0.74 to 0.92, n = 10 over the calibration range). The developed assay proved to be accurate, sensitive and capable of recovering PVP K15 from pharmaceutical formulations. The limits of quantification and detection were calculated statistically as 2.40 and 0.72 mg ml(-1), respectively. Assay reproducibility assessed at five concentrations gave an average coefficient of variance <3.5% and the accuracy of the analytical method was 102.62 +/- 2.04%. The recovery of PVP K15 from directly compressed tablets and Refresh eye drops(TM) was 98.02 +/- 2.73 and 108.35 +/- 6.52%, respectively. (C) 2004 Elsevier B.V. All rights reserved.en
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.subjectpolyvinylpyrrolidone
dc.subjecthigh-performance liquid chromatography
dc.subjectultraviolet detection
dc.subjectformulation
dc.subjectTABLET
dc.subjectFORMULATION
dc.titleDetermination of polyvinylpyrrolidone using high-performance liquid chromatographyen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionPharmaceutics
dc.contributor.institutionSkin and Nail Group
dc.contributor.institutionAirway Group
dc.contributor.institutionBioadhesive Drug Delivery Group
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2004-05-28
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.jpba.2004.01.024
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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