Show simple item record

dc.contributor.authorLiao, Y. H.
dc.contributor.authorBrown, Marc
dc.contributor.authorQuader, A.
dc.contributor.authorMartin, Gary P.
dc.date.accessioned2013-06-25T14:16:48Z
dc.date.available2013-06-25T14:16:48Z
dc.date.issued2003-09
dc.identifier.citationLiao , Y H , Brown , M , Quader , A & Martin , G P 2003 , ' Investigation of the physical properties of spray-dried stabilised lysozyme particles ' Journal of Pharmacy and Pharmacology , vol. 55 , no. 9 , pp. 1213-1221 . https://doi.org/10.1211/0022357021611
dc.identifier.issn0022-3573
dc.identifier.otherPURE: 628743
dc.identifier.otherPURE UUID: 897f3b09-18fa-4d3b-9556-464cec6c94e9
dc.identifier.otherWOS: 000185715500004
dc.identifier.otherScopus: 0141571117
dc.identifier.urihttp://hdl.handle.net/2299/10935
dc.description.abstractThe aim of this study was to investigate the effect of the composition of formulations on the physical properties, including glass-transition temperatures (Tg) and aerodynamic-related characteristics, of spray-dried lysozyme particles. The Tg, as determined by differential scanning calorimetry, of spraydried lysozyme formulations was found to be dependent upon the type and amount of excipient(s) included in the formulation. In addition, the Tg of sucrose-containing particles appeared to be raised markedly by the inclusion of trehalose, but not by dextran. The surfaces of all spray-dried particles were shown by scanning electron microscopy to be smooth with some containing characteristic dimples, typical of spray-dried material, and the morphology appeared to be independent of variation in excipient composition. However, the volume median diameters (VMD) of spray-dried powders, as determined by laser diffraction, were found to depend upon the amounts of excipients. The fine particle fraction of enzyme delivered to the lower stage of a twin-stage impinger from lysozyme-trehalose 1:1 powders appeared to be greater than that from lysozyme-sucrose 1:1 particles (22.5% vs 15.9%) when dispersed via a Rotahaler although a similar dispersibility of the two formulations (39.6% vs 36.7%) was found from a glass inhaler. In general, spray-drying was demonstrated to be feasible to produce respirable particles of the stabilised model protein, with Tg of the formulations being > 30 degreesC higher than room temperature.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofJournal of Pharmacy and Pharmacology
dc.subjectHUMAN DEOXYRIBONUCLEASE RHDNASE
dc.subjectIGE MONOCLONAL-ANTIBODY
dc.subjectFORMULATION EXCIPIENTS
dc.subjectAEROSOL PERFORMANCE
dc.subjectBETA-GALACTOSIDASE
dc.subjectSTORAGE STABILITY
dc.subjectPROTEIN
dc.subjectSUCROSE
dc.subjectPOWDERS
dc.subjectDELIVERY
dc.titleInvestigation of the physical properties of spray-dried stabilised lysozyme particlesen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionPharmaceutics
dc.contributor.institutionSkin and Nail Group
dc.contributor.institutionAirway Group
dc.contributor.institutionBioadhesive Drug Delivery Group
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.versionofrecordhttps://doi.org/10.1211/0022357021611
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstyperestrictedAccess


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record