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dc.contributor.authorKulshreshtha, S.
dc.contributor.authorCoutts, Robert H.A.
dc.date.accessioned2013-06-26T09:01:50Z
dc.date.available2013-06-26T09:01:50Z
dc.date.issued1997
dc.identifier.citationKulshreshtha , S & Coutts , R H A 1997 , ' Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons ' , Journal of Plant Growth Regulation , vol. 22 , no. 2 , pp. 87-92 . https://doi.org/10.1023/A:1005889429586
dc.identifier.issn0167-6903
dc.identifier.otherPURE: 1956344
dc.identifier.otherPURE UUID: fad826c3-65a7-4b17-be7e-82b26bc2ca84
dc.identifier.otherScopus: 0030862091
dc.identifier.urihttp://hdl.handle.net/2299/10957
dc.description.abstractAn in vitro protocol has been developed for direct somatic embryogenesis of zygotic cotyledons from mature sugarbeet (Beta vulgaris L.) embryos. Explants were sequentially cultured on modified Murashige and Skoog (MS) medium supplemented with different combinations of 2,4-D, NAA, BAP and TIBA. Somatic embryogenesis was induced within 4 weeks of culture on embryogenesis induction medium which contained MS medium supplemented with BAP and TIBA. Proliferation of somatic embryos was observed on embryo proliferation medium, which contained MS medium supplemented with BAP and NAA within 4 weeks of culture. Plants were regenerated on hormone free 1/4 strength MS medium containing a low sucrose concentration. With some sugarbeet lines, high frequencies of plant regeneration in excess of 90% were observed. The incorporation of TIBA in the media was essential for successful regeneration.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofJournal of Plant Growth Regulation
dc.titleDirect somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledonsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0030862091&partnerID=8YFLogxK
rioxxterms.versionofrecordhttps://doi.org/10.1023/A:1005889429586
rioxxterms.typeJournal Article/Review


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