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dc.contributor.authorDavies, Keith
dc.contributor.authorAfolabi, P.
dc.contributor.authorO'Shea, P.
dc.date.accessioned2013-06-26T15:17:03Z
dc.date.available2013-06-26T15:17:03Z
dc.date.issued1996-05
dc.identifier.citationDavies , K , Afolabi , P & O'Shea , P 1996 , ' Adhesion of Pasteuria penetrans to the cuticle of root-knot nematodes (Meloidogyne spp.) inhibited by fibronectin : A study of electrostatic and hydrophobic interactions ' , Parasitology , vol. 112 , no. 6 , pp. 553-559 . https://doi.org/10.1017/S0031182000066130
dc.identifier.issn0031-1820
dc.identifier.otherPURE: 590969
dc.identifier.otherPURE UUID: 634c9923-0e29-4901-b17a-1e9f1d11b894
dc.identifier.otherWOS: A1996UQ26400006
dc.identifier.otherScopus: 0030070148
dc.identifier.otherORCID: /0000-0001-6060-2394/work/32215825
dc.identifier.urihttp://hdl.handle.net/2299/10966
dc.description.abstractPasteuria penetrans is a bacterium with the potential to control plant-parasitic nematode populations; the mechanism of spore adhesion, however, is poorly understood. Attachment assays were performed in media supplemented with various concentrations of fibronectin and in the presence and absence of KSCN which modulates hydrophobic interactions. A reduction in the strength of the hydrophobic effect prevented spores from binding to the cuticle as did 20 mu g/ml fibronectin. It was also shown directly utilizing a newly-developed technique which utilizes 3-hexadecanoyl-7-hydrocoumarin as an indicator of the fibronectin binding to the spore surface that the presence of KSCN prohibited binding. This effect was interpreted to indicate that the reduction of binding was the direct result of the influence of hydrophobic interactions between the fibronectin and the spore surface. Western blot analysis of cuticle extracts of 12 Meloidogyne Incognita and Caenorhabditis elegans revealed small amounts of fibronectin to be present. Fibronectin, or a similar receptor, present in the cuticle could be responsible for the adhesion of P. penetrans by hydrophobic interactions.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofParasitology
dc.subjectbacterial adhesion
dc.subjectfibronectin
dc.subjectextracellular matrix
dc.subjectNematoda
dc.subjectMeloidogyne
dc.subjectPasteuria
dc.subjectcuticle
dc.subjectbiological control
dc.subjectelectrostatic potential
dc.subjecthydrophobic surface
dc.subjectCAENORHABDITIS-ELEGANS
dc.subjectPARASITIC NEMATODES
dc.subjectINCOGNITA
dc.subjectPROTEIN
dc.subjectJUVENILES
dc.subjectSEQUENCE
dc.subjectARENARIA
dc.subjectUNC-22
dc.subjectGENE
dc.titleAdhesion of Pasteuria penetrans to the cuticle of root-knot nematodes (Meloidogyne spp.) inhibited by fibronectin : A study of electrostatic and hydrophobic interactionsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1017/S0031182000066130
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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