Show simple item record

dc.contributor.authorMontano, Ximena
dc.contributor.authorLewis, Andrew L.
dc.contributor.authorLeppard, Simon W.
dc.contributor.authorLichtenstein, Conrad
dc.identifier.citationMontano , X , Lewis , A L , Leppard , S W & Lichtenstein , C 2005 , ' Phosphorylcholine is favorable for antibody production from hybridoma cells ' , Biotechnology and Bioengineering , vol. 90 , no. 6 , pp. 770-774 .
dc.identifier.otherPURE: 952322
dc.identifier.otherPURE UUID: 86798c5b-1c29-4eab-bc58-179a7537234b
dc.identifier.otherScopus: 19644396273
dc.description.abstractGrowth of antibody-secreting hybridomas requires special conditions such as serum-free defined media containing growth factors and vitamins. However, the surface on which these cells can proliferate has been shown to play an important role. Phosphorylcholine (PC)-based polymers are zwitterionic compounds with nonbiofouling properties. These polymers are characterized by having reduced protein absorption properties. Our aim was to determine whether well-established hybridoma cell lines were able to proliferate and produce measurable amounts of monoclonal antibodies when grown on PC-polymer-coated surfaces. Comparative experiments using four well-known hybridoma cell lines (PAb421, PAb246, PAb1801 which recognize p53, and PAb280 which recognizes SV40 small t antigen) grown on PC-polymer-coated, uncoated, and two commercially available tissue culture plates showed that PC-polymer-coated plates were more efficient than uncoated plates in sustaining cell growth and monoclonal antibody production/secretion as defined by growth assays and ELISA. Also, results demonstrated that PC-polymer-coated plates were able to perform better than commercially available plates. These observations suggest that PC polymers could be used as an alternative, efficient surface coating to grow hybridoma cell lines and allow detectable antibody secretion. © 2005 Wiley Periodicals, Inc.en
dc.relation.ispartofBiotechnology and Bioengineering
dc.titlePhosphorylcholine is favorable for antibody production from hybridoma cellsen
dc.contributor.institutionParamedic Science
dc.contributor.institutionSchool of Health and Social Work
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAllied Health Professions
dc.contributor.institutionDepartment of Allied Health Professions and Midwifery
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record