Show simple item record

dc.contributor.authorStotz, Henrik
dc.contributor.authorMueller, Stefan
dc.contributor.authorZoeller, Maria
dc.contributor.authorMueller, Martin J.
dc.contributor.authorBerger, Susanne
dc.date.accessioned2013-07-03T10:47:04Z
dc.date.available2013-07-03T10:47:04Z
dc.date.issued2013
dc.identifier.citationStotz , H , Mueller , S , Zoeller , M , Mueller , M J & Berger , S 2013 , ' TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins ' , Journal of Experimental Botany , vol. 64 , no. 4 , pp. 963-975 . https://doi.org/10.1093/jxb/ers389
dc.identifier.issn0022-0957
dc.identifier.otherPURE: 1449542
dc.identifier.otherPURE UUID: 18c9050d-e802-4cd4-8bc5-d49f8f7941cd
dc.identifier.otherScopus: 84874738923
dc.identifier.urihttp://hdl.handle.net/2299/11052
dc.descriptionThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Experimental Botany following peer review. The definitive publisher-authenticated version Vol.64 no.4 pp.963-975 is available at: http://dx.doi.org/10.1093/jxb/ers389
dc.description.abstractJasmonates and phytoprostanes are oxylipins that regulate stress responses and diverse physiological and developmental processes. 12-Oxo-phytodienoic acid (OPDA) and phytoprostanes are structurally related electrophilic cyclopentenones, which activate similar gene expression profiles that are for the most part different from the action of the cyclopentanone jasmonic acid (JA) and its biologically active amino acid conjugates. Whereas JA–isoleucine signals through binding to COI1, the bZIP transcription factors TGA2, TGA5, and TGA6 are involved in regulation of gene expression in response to phytoprostanes. Here root growth inhibition and target gene expression were compared after treatment with JA, OPDA, or phytoprostanes in mutants of the COI1/MYC2 pathway and in different TGA factor mutants. Inhibition of root growth by phytoprostanes was dependent on COI1 but independent of jasmonate biosynthesis. In contrast, phytoprostane-responsive gene expression was strongly dependent on TGA2, TGA5, and TGA6, but not dependent on COI1, MYC2, TGA1, and TGA4. Different mutant and overexpressing lines were used to determine individual contributions of TGA factors to cyclopentenone-responsive gene expression. Whereas OPDAinduced expression of the cytochrome P450 gene CYP81D11 was primarily regulated by TGA2 and TGA5, the glutathione S-transferase gene GST25 and the OPDA reductase gene OPR1 were regulated by TGA5 and TGA6, but less so by TGA2. These results support the model that phytoprostanes and OPDA regulate differently (i) growth responses, which are COI1 dependent but jasmonate independent; and (ii) lipid stress responses, which are strongly dependent on TGA2, TGA5, and TGA6. Identification of molecular components in cyclopentenone signalling provides an insight into novel oxylipin signal transduction pathways.en
dc.language.isoeng
dc.relation.ispartofJournal of Experimental Botany
dc.rightsEmbargoed
dc.titleTGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipinsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.date.embargoedUntil2014-01-01
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Accepted Version
dcterms.dateAccepted2013
rioxxterms.versionAM
rioxxterms.versionofrecordhttps://doi.org/10.1093/jxb/ers389
rioxxterms.licenseref.startdate2014-01-01
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.date.embargo2014-01-01
herts.rights.accesstypeEmbargoed


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record