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dc.contributor.authorKeating, Christopher
dc.contributor.authorPelegrin, P.
dc.contributor.authorMartínez, C.M.
dc.contributor.authorGrundy, D.
dc.identifier.citationKeating , C , Pelegrin , P , Martínez , C M & Grundy , D 2011 , ' P2X 7 receptor-dependent intestinal afferent hypersensitivity in a mouse model of postinfectious irritable bowel syndrome ' , Journal of Immunology , vol. 187 , no. 3 , pp. 1467-1474 .
dc.identifier.otherPURE: 1906244
dc.identifier.otherPURE UUID: 7f89c797-61a2-4b86-b324-e5a1cf02c062
dc.identifier.otherScopus: 80051636256
dc.description.abstractThe ATP-gated P2X7 receptor (P2X7R) was shown to be an important mediator of inflammation and inflammatory pain through its regulation of IL-1β processing and release. Trichinella spiralis-infected mice develop a postinflammatory visceral hypersensitivity that is reminiscent of the clinical features associated with postinfectious irritable bowel syndrome. In this study, we used P2X7R knockout mice (P2X7R-/-) to investigate the role of P2X7R activation in the in vivo production of IL-1β and the development of postinflammatory visceral hypersensitivity in the T. spiralis-infected mouse. During acute nematode infection, IL-1β-containing cells and P2X7R expression were increased in the jejunum of wild-type (WT) mice. Peritoneal and serum IL-1β levels were also increased, which was indicative of elevated IL-1β release. However, in the P2X7R-/- animals, we found that infection had no effect upon intracellular, plasma, or peritoneal IL-1β levels. Conversely, infection augmented peritoneal TNF-α levels in both WT and P2X7R-/- animals. Infection was also associated with a P2X7R-dependent increase in extracellular peritoneal lactate dehydrogenase, and it triggered immunological changes in both strains. Jejunal afferent fiber mechanosensitivity was assessed in uninfected and postinfected WT and P2X7R-/- animals. Postinfected WT animals developed an augmented afferent fiber response to mechanical stimuli; however, this did not develop in postinfected P2X7R-/- animals. Therefore, our results demonstrated that P2X7Rs play a pivotal role in intestinal inflammation and are a trigger for the development of visceral hypersensitivity.en
dc.relation.ispartofJournal of Immunology
dc.titleP2X 7 receptor-dependent intestinal afferent hypersensitivity in a mouse model of postinfectious irritable bowel syndromeen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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