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        S1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B : Identification of regions of potential alternative secondary structure and regulatory function

        Author
        Sunter, G.
        Buck, K.W.
        Coutts, Robert H.A.
        Attention
        2299/11117
        Abstract
        The sensitivity of the supercoiled double-stranded form of the DNA of tanato golden mosaic virus (TGMV), a geminivirus, to the single-strand specific enzyme S1 nuclease has been demonstrated. Specific S1 cleavage sites were identified in TGMV DNA component B by cloning into the single-strand bacteriophage vector M13 mp8 and sequencing of the inserted DNA. Analysis of the DNA sequence at the sites of S1 sensitivity in TGMV DNA component B revealed several possible regions of alternative secondary structure which were clustered in an intergenic region upstream of the starts of the two major open reading frames which are in opposite orientations. This region contains putative transcriptional promoter and modulatory sequences and a possible replication origin. The extreme S1 sensitivity of the supercoiled form of TGMV DNA component A precluded its cloning under the conditions employed for selective cleavage of DNA component B.
        Publication date
        1985-07-11
        Published in
        Nucleic Acids Research
        Published version
        https://doi.org/10.1093/nar/13.13.4645
        Other links
        http://hdl.handle.net/2299/11117
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