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dc.contributor.authorSunter, G.
dc.contributor.authorBuck, K.W.
dc.contributor.authorCoutts, Robert H.A.
dc.date.accessioned2013-07-22T11:32:41Z
dc.date.available2013-07-22T11:32:41Z
dc.date.issued1985-07-11
dc.identifier.citationSunter , G , Buck , K W & Coutts , R H A 1985 , ' S1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B : Identification of regions of potential alternative secondary structure and regulatory function ' , Nucleic Acids Research , vol. 13 , no. 13 , pp. 4645-4659 . https://doi.org/10.1093/nar/13.13.4645
dc.identifier.issn0305-1048
dc.identifier.otherPURE: 1956157
dc.identifier.otherPURE UUID: 1906475f-61e9-45b4-920e-3faa632b64f3
dc.identifier.otherScopus: 0022423462
dc.identifier.urihttp://hdl.handle.net/2299/11117
dc.description.abstractThe sensitivity of the supercoiled double-stranded form of the DNA of tanato golden mosaic virus (TGMV), a geminivirus, to the single-strand specific enzyme S1 nuclease has been demonstrated. Specific S1 cleavage sites were identified in TGMV DNA component B by cloning into the single-strand bacteriophage vector M13 mp8 and sequencing of the inserted DNA. Analysis of the DNA sequence at the sites of S1 sensitivity in TGMV DNA component B revealed several possible regions of alternative secondary structure which were clustered in an intergenic region upstream of the starts of the two major open reading frames which are in opposite orientations. This region contains putative transcriptional promoter and modulatory sequences and a possible replication origin. The extreme S1 sensitivity of the supercoiled form of TGMV DNA component A precluded its cloning under the conditions employed for selective cleavage of DNA component B.en
dc.format.extent15
dc.language.isoeng
dc.relation.ispartofNucleic Acids Research
dc.titleS1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B : Identification of regions of potential alternative secondary structure and regulatory functionen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0022423462&partnerID=8YFLogxK
rioxxterms.versionofrecordhttps://doi.org/10.1093/nar/13.13.4645
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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